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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 2367 | 3' | -48.6 | NC_001416.1 | + | 8017 | 0.66 | 0.957538 |
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Target: 5'- aGCAaaacGGCGGUgcCUGGUGCcgccGCAAUGGCc -3' miRNA: 3'- gCGU----CUGCUA--GACCAUG----CGUUAUUGc -5' |
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| 2367 | 3' | -48.6 | NC_001416.1 | + | 10606 | 0.66 | 0.965418 |
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Target: 5'- aGuCAGGCGucauuuuUCUGGUACGgaaAGUGAUGc -3' miRNA: 3'- gC-GUCUGCu------AGACCAUGCg--UUAUUGC- -5' |
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| 2367 | 3' | -48.6 | NC_001416.1 | + | 10916 | 0.67 | 0.953171 |
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Target: 5'- gCGguGGCGA-CcGGUGCGC--UGGCGu -3' miRNA: 3'- -GCguCUGCUaGaCCAUGCGuuAUUGC- -5' |
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| 2367 | 3' | -48.6 | NC_001416.1 | + | 3854 | 0.68 | 0.920694 |
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Target: 5'- uGCAGACGGcUCaGGaUACG-GAUAACGg -3' miRNA: 3'- gCGUCUGCU-AGaCC-AUGCgUUAUUGC- -5' |
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| 2367 | 3' | -48.6 | NC_001416.1 | + | 15875 | 0.69 | 0.877333 |
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Target: 5'- gCGCAGACGGUCgauGUuugcagACGUAAUGguGCGg -3' miRNA: 3'- -GCGUCUGCUAGac-CA------UGCGUUAU--UGC- -5' |
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| 2367 | 3' | -48.6 | NC_001416.1 | + | 10543 | 0.7 | 0.851804 |
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Target: 5'- gGUAGGCGAUCUGGU-CGU--UGAUu -3' miRNA: 3'- gCGUCUGCUAGACCAuGCGuuAUUGc -5' |
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| 2367 | 3' | -48.6 | NC_001416.1 | + | 11532 | 0.78 | 0.408617 |
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Target: 5'- aGCAGACGAcaUCUGGaaucUGCGCAAgGAUGa -3' miRNA: 3'- gCGUCUGCU--AGACC----AUGCGUUaUUGC- -5' |
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| 2367 | 3' | -48.6 | NC_001416.1 | + | 3008 | 1.11 | 0.003303 |
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Target: 5'- gCGCAGACGAUCUGGUACGCAAUAACGg -3' miRNA: 3'- -GCGUCUGCUAGACCAUGCGUUAUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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