Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2367 | 5' | -54.7 | NC_001416.1 | + | 2592 | 0.68 | 0.549082 |
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Target: 5'- cAGCGCacugagAUCCAGCUGCcagCGGGAa-- -3' miRNA: 3'- -UUGCGg-----UAGGUCGACGua-GUCCUagu -5' |
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| 2367 | 5' | -54.7 | NC_001416.1 | + | 3045 | 1.06 | 0.001134 |
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Target: 5'- cAACGCCAUCCAGCUGCAUCAGGAUCAu -3' miRNA: 3'- -UUGCGGUAGGUCGACGUAGUCCUAGU- -5' |
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| 2367 | 5' | -54.7 | NC_001416.1 | + | 3791 | 0.66 | 0.639816 |
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Target: 5'- cAGCGCCgGUCCGGCUGgG--AGGcgCAa -3' miRNA: 3'- -UUGCGG-UAGGUCGACgUagUCCuaGU- -5' |
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| 2367 | 5' | -54.7 | NC_001416.1 | + | 7212 | 0.66 | 0.646669 |
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Target: 5'- aAGgGCCAUgauuacgCCAGUUGUacggacacgaacucAUCAGGGUCAg -3' miRNA: 3'- -UUgCGGUA-------GGUCGACG--------------UAGUCCUAGU- -5' |
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| 2367 | 5' | -54.7 | NC_001416.1 | + | 7819 | 0.69 | 0.452412 |
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Target: 5'- cACGCCcuGUCCGGCauaGCugauauuuUCAGGGUCAu -3' miRNA: 3'- uUGCGG--UAGGUCGa--CGu-------AGUCCUAGU- -5' |
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| 2367 | 5' | -54.7 | NC_001416.1 | + | 8502 | 0.67 | 0.560271 |
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Target: 5'- aAACGCCGUggUCAGCgGCAccgccaUCGGGAUUu -3' miRNA: 3'- -UUGCGGUA--GGUCGaCGU------AGUCCUAGu -5' |
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| 2367 | 5' | -54.7 | NC_001416.1 | + | 10797 | 0.66 | 0.651235 |
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Target: 5'- cGugGCUgAUCCuGCUGCAaCAGGGggggCAg -3' miRNA: 3'- -UugCGG-UAGGuCGACGUaGUCCUa---GU- -5' |
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| 2367 | 5' | -54.7 | NC_001416.1 | + | 10875 | 0.66 | 0.662634 |
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Target: 5'- -cUGCCcccCCuGUUGCAgCAGGAUCAg -3' miRNA: 3'- uuGCGGua-GGuCGACGUaGUCCUAGU- -5' |
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| 2367 | 5' | -54.7 | NC_001416.1 | + | 16094 | 0.68 | 0.537964 |
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Target: 5'- cGGCGUCAUCCuGC-GCAUCcGGAUa- -3' miRNA: 3'- -UUGCGGUAGGuCGaCGUAGuCCUAgu -5' |
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| 2367 | 5' | -54.7 | NC_001416.1 | + | 20025 | 0.68 | 0.537964 |
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Target: 5'- cGAUGCCAgugcaUCAGCUGC-UCAGG-UCGc -3' miRNA: 3'- -UUGCGGUa----GGUCGACGuAGUCCuAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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