Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23673 | 5' | -57.3 | NC_005261.1 | + | 22081 | 0.66 | 0.842942 |
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Target: 5'- cUCGAGGGGGcgCCgC-GUGgggGcGGGAGGg -3' miRNA: 3'- aAGCUCCUCUa-GG-GaCACa--C-CCCUCC- -5' |
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| 23673 | 5' | -57.3 | NC_005261.1 | + | 42972 | 0.66 | 0.826414 |
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Target: 5'- cUUCaAGGAGAUCCUgcUG-GGGGAGc -3' miRNA: 3'- -AAGcUCCUCUAGGGacACaCCCCUCc -5' |
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| 23673 | 5' | -57.3 | NC_005261.1 | + | 32778 | 0.67 | 0.809181 |
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Target: 5'- --gGAGGAGAgggaggCCCg--GcUGGGGGGGc -3' miRNA: 3'- aagCUCCUCUa-----GGGacaC-ACCCCUCC- -5' |
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| 23673 | 5' | -57.3 | NC_005261.1 | + | 135822 | 0.67 | 0.809181 |
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Target: 5'- gUUCGAGGAGuacAUCCCUGgggcuugcGUGGcGGGc- -3' miRNA: 3'- -AAGCUCCUC---UAGGGACa-------CACC-CCUcc -5' |
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| 23673 | 5' | -57.3 | NC_005261.1 | + | 27238 | 0.67 | 0.80032 |
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Target: 5'- --gGGGGAGG-CgCCUGggGUGGGGcGGGg -3' miRNA: 3'- aagCUCCUCUaG-GGACa-CACCCC-UCC- -5' |
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| 23673 | 5' | -57.3 | NC_005261.1 | + | 111080 | 0.68 | 0.73454 |
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Target: 5'- aUCGAGGg---CCUgGUGUGGGGGGc -3' miRNA: 3'- aAGCUCCucuaGGGaCACACCCCUCc -5' |
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| 23673 | 5' | -57.3 | NC_005261.1 | + | 24268 | 0.68 | 0.724715 |
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Target: 5'- -gCGGGGAGGgggcuugCCCgg-GUGGGGGuGGg -3' miRNA: 3'- aaGCUCCUCUa------GGGacaCACCCCU-CC- -5' |
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| 23673 | 5' | -57.3 | NC_005261.1 | + | 29117 | 0.69 | 0.684711 |
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Target: 5'- -cCGAGGGGAgCCCUGgcgccGGGGGGc -3' miRNA: 3'- aaGCUCCUCUaGGGACaca--CCCCUCc -5' |
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| 23673 | 5' | -57.3 | NC_005261.1 | + | 34173 | 1.08 | 0.002102 |
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Target: 5'- cUUCGAGGAGAUCCCUGUGUGGGGAGGg -3' miRNA: 3'- -AAGCUCCUCUAGGGACACACCCCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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