Results 61 - 67 of 67 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23674 | 3' | -54 | NC_005261.1 | + | 128070 | 0.68 | 0.893421 |
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Target: 5'- uGGCGGgcGGGCGGgcGAGCgagcggguuaaCGGGCGa -3' miRNA: 3'- gCCGUUa-UCCGUCuaCUCGa----------GCCUGC- -5' |
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| 23674 | 3' | -54 | NC_005261.1 | + | 128886 | 0.68 | 0.872034 |
|
Target: 5'- aGGCGG-GGGcCGGGUGGGaggCGGGCGu -3' miRNA: 3'- gCCGUUaUCC-GUCUACUCga-GCCUGC- -5' |
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| 23674 | 3' | -54 | NC_005261.1 | + | 129606 | 0.68 | 0.879394 |
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Target: 5'- gCGGCAAUuucGCGGcgGGG-UCGGGCGc -3' miRNA: 3'- -GCCGUUAuc-CGUCuaCUCgAGCCUGC- -5' |
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| 23674 | 3' | -54 | NC_005261.1 | + | 129735 | 0.67 | 0.906495 |
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Target: 5'- gCGGCA--GGGCGGcgGGGCcCGGcCu -3' miRNA: 3'- -GCCGUuaUCCGUCuaCUCGaGCCuGc -5' |
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| 23674 | 3' | -54 | NC_005261.1 | + | 135093 | 0.67 | 0.906495 |
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Target: 5'- gCGGCcc--GGCGGcgGAGCgcgaggCGGACc -3' miRNA: 3'- -GCCGuuauCCGUCuaCUCGa-----GCCUGc -5' |
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| 23674 | 3' | -54 | NC_005261.1 | + | 136565 | 0.68 | 0.893421 |
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Target: 5'- gGGCAGaAGGCgcGGGUGGGC--GGGCGc -3' miRNA: 3'- gCCGUUaUCCG--UCUACUCGagCCUGC- -5' |
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| 23674 | 3' | -54 | NC_005261.1 | + | 138204 | 0.69 | 0.848634 |
|
Target: 5'- gGGCGggGGGCAGGgcgGAcggcgGCUCGGcCGc -3' miRNA: 3'- gCCGUuaUCCGUCUa--CU-----CGAGCCuGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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