miRNA: 3'- ugaauaaCUCAaaaCACGCUUGCGac -5'
miRNA: 3'- -UGAAUAACUCaaaACACGCUUGcGAC- -5'
miRNA: 3'- ugAAUAACUCaAAACACGCUUGcGAC- -5'
miRNA: 3'- -UGAAUAACUCAAAACACGCUUGCGAC- -5'
miRNA: 3'- -UGAAUAACUCAAAACACGCUUGCGAC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23677 | 5' | -44.2 | NC_005261.1 | + | 134613 | 0.66 | 0.999995 |
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Target: 5'- ------cGGGUcugGUGCGAACGCg- -3' miRNA: 3'- ugaauaaCUCAaaaCACGCUUGCGac -5' |
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| 23677 | 5' | -44.2 | NC_005261.1 | + | 8946 | 0.8 | 0.865 |
|
Target: 5'- uACUUAUUGAGcucUGUGCGAACaCUGg -3' miRNA: 3'- -UGAAUAACUCaaaACACGCUUGcGAC- -5' |
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| 23677 | 5' | -44.2 | NC_005261.1 | + | 24812 | 0.8 | 0.865 |
|
Target: 5'- -aUUAUUGAGcUUUGUGCGAACaCUGg -3' miRNA: 3'- ugAAUAACUCaAAACACGCUUGcGAC- -5' |
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| 23677 | 5' | -44.2 | NC_005261.1 | + | 24997 | 1.11 | 0.028344 |
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Target: 5'- uACUUAUUGAGUUUUGUGCGAACGCUGg -3' miRNA: 3'- -UGAAUAACUCAAAACACGCUUGCGAC- -5' |
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| 23677 | 5' | -44.2 | NC_005261.1 | + | 25113 | 1.11 | 0.028344 |
|
Target: 5'- uACUUAUUGAGUUUUGUGCGAACGCUGg -3' miRNA: 3'- -UGAAUAACUCAAAACACGCUUGCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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