Results 121 - 128 of 128 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23679 | 5' | -53.8 | NC_005261.1 | + | 108452 | 0.66 | 0.963333 |
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Target: 5'- gAGCAGCgcgUCGuCGGCGAGGAucuccgcguCGGCGc -3' miRNA: 3'- -UUGUUGa--GGCuGCUGCUCCU---------GCUGCu -5' |
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| 23679 | 5' | -53.8 | NC_005261.1 | + | 81220 | 0.66 | 0.963333 |
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Target: 5'- --gGACcaCCGugGcgccCGAGGGCGGCGGg -3' miRNA: 3'- uugUUGa-GGCugCu---GCUCCUGCUGCU- -5' |
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| 23679 | 5' | -53.8 | NC_005261.1 | + | 44014 | 0.66 | 0.963333 |
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Target: 5'- -cCGGCUUCGGCG-CGGcGGGCGGCc- -3' miRNA: 3'- uuGUUGAGGCUGCuGCU-CCUGCUGcu -5' |
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| 23679 | 5' | -53.8 | NC_005261.1 | + | 30833 | 0.66 | 0.963333 |
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Target: 5'- gGACGGggCCGAgGAgGAGGAgGAgGAg -3' miRNA: 3'- -UUGUUgaGGCUgCUgCUCCUgCUgCU- -5' |
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| 23679 | 5' | -53.8 | NC_005261.1 | + | 138056 | 0.66 | 0.963333 |
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Target: 5'- uGGCGGCggCGGCGGCGGcGGCGGCa- -3' miRNA: 3'- -UUGUUGagGCUGCUGCUcCUGCUGcu -5' |
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| 23679 | 5' | -53.8 | NC_005261.1 | + | 90063 | 0.66 | 0.963333 |
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Target: 5'- cAGCGGCgccccgCgGGCGcCGAGGgcaGCGACGGg -3' miRNA: 3'- -UUGUUGa-----GgCUGCuGCUCC---UGCUGCU- -5' |
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| 23679 | 5' | -53.8 | NC_005261.1 | + | 385 | 0.66 | 0.963333 |
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Target: 5'- uGGCGGCggCGGCGGCGGcGGCGGCa- -3' miRNA: 3'- -UUGUUGagGCUGCUGCUcCUGCUGcu -5' |
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| 23679 | 5' | -53.8 | NC_005261.1 | + | 14123 | 0.65 | 0.96567 |
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Target: 5'- -uCGGCUCgGGCGgugggcagucgcucGCGAGGACGcuccagccGCGAa -3' miRNA: 3'- uuGUUGAGgCUGC--------------UGCUCCUGC--------UGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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