Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23681 | 5' | -57 | NC_005261.1 | + | 106598 | 0.66 | 0.822535 |
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Target: 5'- -cGCUCCUCCUGGACcucgucCCAGa -3' miRNA: 3'- uaCGAGGGGGGCCUGaugaaaGGUCa -5' |
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| 23681 | 5' | -57 | NC_005261.1 | + | 5998 | 0.66 | 0.822535 |
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Target: 5'- -cGCUCCUCCCGcGGCgaggGC--UCCGGc -3' miRNA: 3'- uaCGAGGGGGGC-CUGa---UGaaAGGUCa -5' |
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| 23681 | 5' | -57 | NC_005261.1 | + | 91179 | 0.66 | 0.813801 |
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Target: 5'- -aGCUCCCgCCGGGCUucgcGCgcUUCGGc -3' miRNA: 3'- uaCGAGGGgGGCCUGA----UGaaAGGUCa -5' |
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| 23681 | 5' | -57 | NC_005261.1 | + | 74103 | 0.66 | 0.804897 |
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Target: 5'- -cGC-CCCCCCGGGCccggacCCGGUg -3' miRNA: 3'- uaCGaGGGGGGCCUGaugaaaGGUCA- -5' |
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| 23681 | 5' | -57 | NC_005261.1 | + | 36989 | 0.67 | 0.786617 |
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Target: 5'- -gGCUCCagccggagCCCgCGGGCUGCgcggggCCAGUu -3' miRNA: 3'- uaCGAGG--------GGG-GCCUGAUGaaa---GGUCA- -5' |
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| 23681 | 5' | -57 | NC_005261.1 | + | 45677 | 0.67 | 0.767767 |
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Target: 5'- -cGCgCgCCCCGGGCUGCUgaaCGGg -3' miRNA: 3'- uaCGaGgGGGGCCUGAUGAaagGUCa -5' |
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| 23681 | 5' | -57 | NC_005261.1 | + | 133795 | 0.68 | 0.718641 |
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Target: 5'- -cGCaUCCCgCCCGGGCUGCUcgaCGGc -3' miRNA: 3'- uaCG-AGGG-GGGCCUGAUGAaagGUCa -5' |
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| 23681 | 5' | -57 | NC_005261.1 | + | 110174 | 0.69 | 0.677915 |
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Target: 5'- -cGCgCCCCCCGGGCgGCagUUCCGc- -3' miRNA: 3'- uaCGaGGGGGGCCUGaUGa-AAGGUca -5' |
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| 23681 | 5' | -57 | NC_005261.1 | + | 55897 | 0.76 | 0.286258 |
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Target: 5'- -cGcCUCCCUCCGGugUGCUucUUCCAGc -3' miRNA: 3'- uaC-GAGGGGGGCCugAUGA--AAGGUCa -5' |
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| 23681 | 5' | -57 | NC_005261.1 | + | 21294 | 1.06 | 0.002959 |
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Target: 5'- cAUGCUCCCCCCGGACUACUUUCCAGUg -3' miRNA: 3'- -UACGAGGGGGGCCUGAUGAAAGGUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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