Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23682 | 3' | -56.4 | NC_005261.1 | + | 35627 | 0.66 | 0.860643 |
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Target: 5'- ----cGUuuCUGCCCAAGCCCCaGCc- -3' miRNA: 3'- uaguaCAu-GACGGGUUCGGGGgUGcu -5' |
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| 23682 | 3' | -56.4 | NC_005261.1 | + | 27402 | 0.66 | 0.860643 |
|
Target: 5'- -cCAUGUuuuuaUGCaaaCGAGCCCCgACGGc -3' miRNA: 3'- uaGUACAug---ACGg--GUUCGGGGgUGCU- -5' |
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| 23682 | 3' | -56.4 | NC_005261.1 | + | 27263 | 0.67 | 0.782815 |
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Target: 5'- --gGUGUGCgggcaGCCCGgccGGCCCCCggaccgcccucGCGAg -3' miRNA: 3'- uagUACAUGa----CGGGU---UCGGGGG-----------UGCU- -5' |
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| 23682 | 3' | -56.4 | NC_005261.1 | + | 21117 | 1.07 | 0.002667 |
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Target: 5'- cAUCAUGUACUGCCCAAGCCCCCACGAg -3' miRNA: 3'- -UAGUACAUGACGGGUUCGGGGGUGCU- -5' |
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| 23682 | 3' | -56.4 | NC_005261.1 | + | 13187 | 0.66 | 0.827868 |
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Target: 5'- ---cUGUAgU-CCCAGGCCUCCACGu -3' miRNA: 3'- uaguACAUgAcGGGUUCGGGGGUGCu -5' |
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| 23682 | 3' | -56.4 | NC_005261.1 | + | 12720 | 0.68 | 0.744195 |
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Target: 5'- cUCggGcGCgcggGCCCGccGGCCCCCugGGc -3' miRNA: 3'- uAGuaCaUGa---CGGGU--UCGGGGGugCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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