miRNA: 3'- -GGU--ACCAU---UGGUAUCAgUGCac--ACGC- -5'
miRNA: 3'- -GGUACCauUGGUauCaGUGC-ACACGC- -5'
miRNA: 3'- -GGUACCa----UUGGUaUCAGU-GCACaCGC- -5'
miRNA: 3'- -GGUACCAUUGGUAUCAGUGCACACGC- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23683 | 5' | -49 | NC_005261.1 | + | 88743 | 0.69 | 0.98771 |
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Target: 5'- gCCAgcUGGUGgacGCCAUGGUgGCGgcgcUGCGc -3' miRNA: 3'- -GGU--ACCAU---UGGUAUCAgUGCac--ACGC- -5' |
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| 23683 | 5' | -49 | NC_005261.1 | + | 113185 | 0.7 | 0.975112 |
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Target: 5'- gUCAUGGccACCAgcGcCACGcUGUGCGa -3' miRNA: 3'- -GGUACCauUGGUauCaGUGC-ACACGC- -5' |
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| 23683 | 5' | -49 | NC_005261.1 | + | 73706 | 0.71 | 0.954994 |
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Target: 5'- gCCAUGGcgcagGACCGcGGUCGgCGUGcGCGc -3' miRNA: 3'- -GGUACCa----UUGGUaUCAGU-GCACaCGC- -5' |
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| 23683 | 5' | -49 | NC_005261.1 | + | 20393 | 1.13 | 0.007864 |
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Target: 5'- gCCAUGGUAACCAUAGUCACGUGUGCGg -3' miRNA: 3'- -GGUACCAUUGGUAUCAGUGCACACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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