Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23685 | 5' | -57.5 | NC_005261.1 | + | 20835 | 0.66 | 0.845692 |
Target: 5'- -aCUGCGCGUUCGcgGgGGCAG-CGUGg -3' miRNA: 3'- gaGGUGUGCAGGCa-CgCCGUCaGCAU- -5' |
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23685 | 5' | -57.5 | NC_005261.1 | + | 54146 | 0.66 | 0.829313 |
Target: 5'- -gCCGCACGcggCCGUGCGcGgGGUUGc- -3' miRNA: 3'- gaGGUGUGCa--GGCACGC-CgUCAGCau -5' |
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23685 | 5' | -57.5 | NC_005261.1 | + | 74273 | 0.66 | 0.803429 |
Target: 5'- --gCGCGCGUCCGcgGCgggcggGGCGGUCGc- -3' miRNA: 3'- gagGUGUGCAGGCa-CG------CCGUCAGCau -5' |
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23685 | 5' | -57.5 | NC_005261.1 | + | 73785 | 0.67 | 0.794481 |
Target: 5'- -cCUGCgACGUgCGcGCGGCGGUCGg- -3' miRNA: 3'- gaGGUG-UGCAgGCaCGCCGUCAGCau -5' |
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23685 | 5' | -57.5 | NC_005261.1 | + | 29989 | 0.67 | 0.794481 |
Target: 5'- -cCgGCGCGUCCGgcaGCGGCGcGUCu-- -3' miRNA: 3'- gaGgUGUGCAGGCa--CGCCGU-CAGcau -5' |
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23685 | 5' | -57.5 | NC_005261.1 | + | 75467 | 0.67 | 0.794481 |
Target: 5'- uUCCAgGCG-CCGgGCGGCGGcgCGg- -3' miRNA: 3'- gAGGUgUGCaGGCaCGCCGUCa-GCau -5' |
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23685 | 5' | -57.5 | NC_005261.1 | + | 111664 | 0.67 | 0.785387 |
Target: 5'- cCUCC-CGCG-CCGcgGUGGCGG-CGUAc -3' miRNA: 3'- -GAGGuGUGCaGGCa-CGCCGUCaGCAU- -5' |
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23685 | 5' | -57.5 | NC_005261.1 | + | 94096 | 0.67 | 0.776156 |
Target: 5'- -gCCGC-CGUCCGUGCGGaAGcacaCGUGg -3' miRNA: 3'- gaGGUGuGCAGGCACGCCgUCa---GCAU- -5' |
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23685 | 5' | -57.5 | NC_005261.1 | + | 54480 | 0.67 | 0.766798 |
Target: 5'- aUCCGgGCGUCgG-GCGGCAGgCGc- -3' miRNA: 3'- gAGGUgUGCAGgCaCGCCGUCaGCau -5' |
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23685 | 5' | -57.5 | NC_005261.1 | + | 31337 | 0.67 | 0.75732 |
Target: 5'- -gCCAgCGCG-CCGcUGCGGCGGcgCGUGg -3' miRNA: 3'- gaGGU-GUGCaGGC-ACGCCGUCa-GCAU- -5' |
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23685 | 5' | -57.5 | NC_005261.1 | + | 81302 | 0.69 | 0.678303 |
Target: 5'- --gCGCACGUCCGUGCcGUuGUCGg- -3' miRNA: 3'- gagGUGUGCAGGCACGcCGuCAGCau -5' |
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23685 | 5' | -57.5 | NC_005261.1 | + | 102667 | 0.69 | 0.63658 |
Target: 5'- cCUCCGCggcgcccagcgccGCGUCCGccGCGGCcGUCGc- -3' miRNA: 3'- -GAGGUG-------------UGCAGGCa-CGCCGuCAGCau -5' |
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23685 | 5' | -57.5 | NC_005261.1 | + | 114636 | 0.71 | 0.526705 |
Target: 5'- gUCaCGCGCG-CCGcGCGGCAGUCa-- -3' miRNA: 3'- gAG-GUGUGCaGGCaCGCCGUCAGcau -5' |
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23685 | 5' | -57.5 | NC_005261.1 | + | 86970 | 0.74 | 0.381139 |
Target: 5'- --gCACACGUCCG-GCGGCAGcacggcgcccUCGUGg -3' miRNA: 3'- gagGUGUGCAGGCaCGCCGUC----------AGCAU- -5' |
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23685 | 5' | -57.5 | NC_005261.1 | + | 19309 | 1.07 | 0.002675 |
Target: 5'- gCUCCACACGUCCGUGCGGCAGUCGUAg -3' miRNA: 3'- -GAGGUGUGCAGGCACGCCGUCAGCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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