Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2369 | 5' | -54.8 | NC_001416.1 | + | 3609 | 1.07 | 0.001034 |
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Target: 5'- gAUGCUCGACACGCUGCAGAACACGCAg -3' miRNA: 3'- -UACGAGCUGUGCGACGUCUUGUGCGU- -5' |
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| 2369 | 5' | -54.8 | NC_001416.1 | + | 16514 | 0.73 | 0.275023 |
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Target: 5'- cUGCUCGGCgauGCGCUGUaugccgguauGGAACGgGCAg -3' miRNA: 3'- uACGAGCUG---UGCGACG----------UCUUGUgCGU- -5' |
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| 2369 | 5' | -54.8 | NC_001416.1 | + | 16281 | 0.71 | 0.363388 |
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Target: 5'- -aGUUCGacgGCAC-CUGCAGAAUACGCc -3' miRNA: 3'- uaCGAGC---UGUGcGACGUCUUGUGCGu -5' |
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| 2369 | 5' | -54.8 | NC_001416.1 | + | 9833 | 0.71 | 0.369635 |
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Target: 5'- gAUGCUCaAUGCGCUGCAGGGCugacaguucagaaaGCGUg -3' miRNA: 3'- -UACGAGcUGUGCGACGUCUUG--------------UGCGu -5' |
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| 2369 | 5' | -54.8 | NC_001416.1 | + | 24162 | 0.69 | 0.480027 |
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Target: 5'- cGUGCUgCGGCugGCUGguGAACuucCGa- -3' miRNA: 3'- -UACGA-GCUGugCGACguCUUGu--GCgu -5' |
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| 2369 | 5' | -54.8 | NC_001416.1 | + | 44378 | 0.68 | 0.533771 |
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Target: 5'- -aGCgCGAUAUGCUGCGcuuGCugGCAu -3' miRNA: 3'- uaCGaGCUGUGCGACGUcu-UGugCGU- -5' |
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| 2369 | 5' | -54.8 | NC_001416.1 | + | 11328 | 0.68 | 0.555878 |
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Target: 5'- -cGC-CGACACGUUaUGGAACugGCGa -3' miRNA: 3'- uaCGaGCUGUGCGAcGUCUUGugCGU- -5' |
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| 2369 | 5' | -54.8 | NC_001416.1 | + | 2873 | 0.66 | 0.668807 |
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Target: 5'- -gGCaUGACAuCGCUGCGcGAAUAUGCc -3' miRNA: 3'- uaCGaGCUGU-GCGACGU-CUUGUGCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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