Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23693 | 3' | -54.9 | NC_005261.1 | + | 23525 | 0.66 | 0.937728 |
Target: 5'- aCGCG-GUCGGgcgagCGGCa-GAGGCGG-Ca -3' miRNA: 3'- -GCGCaCAGCUa----GCCGagCUUCGUCaG- -5' |
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23693 | 3' | -54.9 | NC_005261.1 | + | 109845 | 0.66 | 0.937728 |
Target: 5'- gGCGUGcCGuugguggCGGCUCcugGggGCAG-Cg -3' miRNA: 3'- gCGCACaGCua-----GCCGAG---CuuCGUCaG- -5' |
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23693 | 3' | -54.9 | NC_005261.1 | + | 23634 | 0.66 | 0.93326 |
Target: 5'- gGCGgggGUCGggCGGCUgGAAGggagaggggaggagaGGUCg -3' miRNA: 3'- gCGCa--CAGCuaGCCGAgCUUCg--------------UCAG- -5' |
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23693 | 3' | -54.9 | NC_005261.1 | + | 96828 | 0.66 | 0.932752 |
Target: 5'- aGC-UGUCGG-CGGUgUCGAGGCcggGGUCg -3' miRNA: 3'- gCGcACAGCUaGCCG-AGCUUCG---UCAG- -5' |
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23693 | 3' | -54.9 | NC_005261.1 | + | 13694 | 0.66 | 0.932752 |
Target: 5'- gGCGUGgggCGGggcgGGCUgGggGCGGg- -3' miRNA: 3'- gCGCACa--GCUag--CCGAgCuuCGUCag -5' |
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23693 | 3' | -54.9 | NC_005261.1 | + | 13074 | 0.66 | 0.927533 |
Target: 5'- gCGCGUccgccggcccGUCGGcgggcggcgggcUCGGCUCGggGgC-GUCg -3' miRNA: 3'- -GCGCA----------CAGCU------------AGCCGAGCuuC-GuCAG- -5' |
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23693 | 3' | -54.9 | NC_005261.1 | + | 43169 | 0.66 | 0.927533 |
Target: 5'- uCGCGgcgCGAUaCGGCgccgccggCGAGGCAGa- -3' miRNA: 3'- -GCGCacaGCUA-GCCGa-------GCUUCGUCag -5' |
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23693 | 3' | -54.9 | NC_005261.1 | + | 108673 | 0.66 | 0.916364 |
Target: 5'- uCGCGUccucGUCGucCGGCUCGggGaAGUa -3' miRNA: 3'- -GCGCA----CAGCuaGCCGAGCuuCgUCAg -5' |
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23693 | 3' | -54.9 | NC_005261.1 | + | 48442 | 0.67 | 0.884241 |
Target: 5'- uCGCGgugcGUgGcgCGGC-CGgcGCGGUCa -3' miRNA: 3'- -GCGCa---CAgCuaGCCGaGCuuCGUCAG- -5' |
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23693 | 3' | -54.9 | NC_005261.1 | + | 24385 | 0.68 | 0.869774 |
Target: 5'- gGUGggcUCG-UCGGCggCGggGCGGUCc -3' miRNA: 3'- gCGCac-AGCuAGCCGa-GCuuCGUCAG- -5' |
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23693 | 3' | -54.9 | NC_005261.1 | + | 115467 | 0.68 | 0.84646 |
Target: 5'- cCGCcucgGUGUCGGUCgcgGGCUCGGccgcGGCGG-Cg -3' miRNA: 3'- -GCG----CACAGCUAG---CCGAGCU----UCGUCaG- -5' |
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23693 | 3' | -54.9 | NC_005261.1 | + | 120293 | 0.69 | 0.821364 |
Target: 5'- gCGCGUGuucuUCGAg-GGCggCGAGGCuGUCu -3' miRNA: 3'- -GCGCAC----AGCUagCCGa-GCUUCGuCAG- -5' |
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23693 | 3' | -54.9 | NC_005261.1 | + | 34690 | 0.69 | 0.821364 |
Target: 5'- uGCGUGaCGGUgGGCcccucgcaccUCGAgccucAGCGGUCg -3' miRNA: 3'- gCGCACaGCUAgCCG----------AGCU-----UCGUCAG- -5' |
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23693 | 3' | -54.9 | NC_005261.1 | + | 13135 | 0.69 | 0.821364 |
Target: 5'- gCGCGUGU-GGUCGGUgacggCGcgcaccAGGCGGUCc -3' miRNA: 3'- -GCGCACAgCUAGCCGa----GC------UUCGUCAG- -5' |
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23693 | 3' | -54.9 | NC_005261.1 | + | 4314 | 0.7 | 0.736579 |
Target: 5'- cCGCGUagCGcgCGGCcgccucgcggaucUCGggGCAGUCc -3' miRNA: 3'- -GCGCAcaGCuaGCCG-------------AGCuuCGUCAG- -5' |
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23693 | 3' | -54.9 | NC_005261.1 | + | 74273 | 0.74 | 0.553793 |
Target: 5'- gCGCGcGUCcg-CGGCgggCGggGCGGUCg -3' miRNA: 3'- -GCGCaCAGcuaGCCGa--GCuuCGUCAG- -5' |
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23693 | 3' | -54.9 | NC_005261.1 | + | 16206 | 0.76 | 0.428887 |
Target: 5'- cCGCGUGauggccUCGAggcUCGGCcagcccUCGggGCGGUCg -3' miRNA: 3'- -GCGCAC------AGCU---AGCCG------AGCuuCGUCAG- -5' |
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23693 | 3' | -54.9 | NC_005261.1 | + | 14098 | 0.78 | 0.352781 |
Target: 5'- uCGCGgucGUCGAggcagacgggcgUCGGCUCGGgcggugGGCAGUCg -3' miRNA: 3'- -GCGCa--CAGCU------------AGCCGAGCU------UCGUCAG- -5' |
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23693 | 3' | -54.9 | NC_005261.1 | + | 11986 | 1.09 | 0.003469 |
Target: 5'- cCGCGUGUCGAUCGGCUCGAAGCAGUCc -3' miRNA: 3'- -GCGCACAGCUAGCCGAGCUUCGUCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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