Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23696 | 3' | -54.2 | NC_005261.1 | + | 108670 | 0.71 | 0.79336 |
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Target: 5'- gCgUCG-CGUCCUCGUCGuccggcUCGGGGa-- -3' miRNA: 3'- -GgAGCaGCAGGAGCAGC------AGCCUCaac -5' |
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| 23696 | 3' | -54.2 | NC_005261.1 | + | 16390 | 0.72 | 0.746704 |
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Target: 5'- gCCUCGcCGUCgUCGUCGcCGGgcGGUg- -3' miRNA: 3'- -GGAGCaGCAGgAGCAGCaGCC--UCAac -5' |
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| 23696 | 3' | -54.2 | NC_005261.1 | + | 22860 | 0.72 | 0.717448 |
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Target: 5'- --aCGUCGUCCUCGcCGUCcGAGaUGg -3' miRNA: 3'- ggaGCAGCAGGAGCaGCAGcCUCaAC- -5' |
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| 23696 | 3' | -54.2 | NC_005261.1 | + | 12787 | 0.73 | 0.687543 |
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Target: 5'- gCUCGUCGgcgagCUCGUCGUCGGcGUc- -3' miRNA: 3'- gGAGCAGCag---GAGCAGCAGCCuCAac -5' |
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| 23696 | 3' | -54.2 | NC_005261.1 | + | 32605 | 0.77 | 0.487868 |
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Target: 5'- uCCUCGUCGUCCgCGUCcUCGGAc--- -3' miRNA: 3'- -GGAGCAGCAGGaGCAGcAGCCUcaac -5' |
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| 23696 | 3' | -54.2 | NC_005261.1 | + | 10771 | 0.84 | 0.203587 |
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Target: 5'- uCCUCGUCGUCCUCGUCGUCa------ -3' miRNA: 3'- -GGAGCAGCAGGAGCAGCAGccucaac -5' |
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| 23696 | 3' | -54.2 | NC_005261.1 | + | 10816 | 1.1 | 0.004733 |
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Target: 5'- uCCUCGUCGUCCUCGUCGUCGGAGUUGu -3' miRNA: 3'- -GGAGCAGCAGGAGCAGCAGCCUCAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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