Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 237 | 5' | -51.6 | AC_000008.1 | + | 18909 | 0.66 | 0.799402 |
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Target: 5'- -uGCAGuuuGCCCGCGCcACCgagacGUACUu -3' miRNA: 3'- ucUGUCuu-UGGGCGCGaUGG-----UAUGAc -5' |
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| 237 | 5' | -51.6 | AC_000008.1 | + | 20441 | 0.67 | 0.723393 |
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Target: 5'- -uGCu--GGCCUGCGCUACCGcucaauguUGCUGg -3' miRNA: 3'- ucUGucuUUGGGCGCGAUGGU--------AUGAC- -5' |
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| 237 | 5' | -51.6 | AC_000008.1 | + | 17581 | 0.68 | 0.677387 |
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Target: 5'- gAGGCAG-GACCCugGUGCUGCCA-ACa- -3' miRNA: 3'- -UCUGUCuUUGGG--CGCGAUGGUaUGac -5' |
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| 237 | 5' | -51.6 | AC_000008.1 | + | 27774 | 0.68 | 0.677387 |
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Target: 5'- -uGCAGGAuguacaGCCCGUgcaccGCUGCCAgUACUGc -3' miRNA: 3'- ucUGUCUU------UGGGCG-----CGAUGGU-AUGAC- -5' |
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| 237 | 5' | -51.6 | AC_000008.1 | + | 1085 | 0.69 | 0.595474 |
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Target: 5'- uGGCGGGGAaguggCCGCGCUGguCCGUGCa- -3' miRNA: 3'- uCUGUCUUUg----GGCGCGAU--GGUAUGac -5' |
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| 237 | 5' | -51.6 | AC_000008.1 | + | 15894 | 0.7 | 0.53777 |
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Target: 5'- cAGGCuGggGCCUGCGCUucCCAaGCa- -3' miRNA: 3'- -UCUGuCuuUGGGCGCGAu-GGUaUGac -5' |
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| 237 | 5' | -51.6 | AC_000008.1 | + | 31908 | 0.71 | 0.504066 |
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Target: 5'- aGGGCAGAAACCUGCugGCgcagauCCAaagcUGCUGa -3' miRNA: 3'- -UCUGUCUUUGGGCG--CGau----GGU----AUGAC- -5' |
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| 237 | 5' | -51.6 | AC_000008.1 | + | 8664 | 0.73 | 0.388786 |
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Target: 5'- cGGGCAGGAGCUgGUGCUGCgCGcguagguUGCUGg -3' miRNA: 3'- -UCUGUCUUUGGgCGCGAUG-GU-------AUGAC- -5' |
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| 237 | 5' | -51.6 | AC_000008.1 | + | 18553 | 0.78 | 0.193949 |
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Target: 5'- -aGCAGAAACCUGUGCUGCCAgGCc- -3' miRNA: 3'- ucUGUCUUUGGGCGCGAUGGUaUGac -5' |
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| 237 | 5' | -51.6 | AC_000008.1 | + | 1960 | 1.1 | 0.001085 |
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Target: 5'- gAGACAGAAACCCGCGCUACCAUACUGg -3' miRNA: 3'- -UCUGUCUUUGGGCGCGAUGGUAUGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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