Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23704 | 5' | -55.5 | NC_005261.1 | + | 16404 | 0.66 | 0.90329 |
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Target: 5'- gUCgCCGGgcggugcgGGAGAGGcCCCCUCGAc- -3' miRNA: 3'- gAG-GGCUa-------CCUCUCUuGGGGGGUUuu -5' |
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| 23704 | 5' | -55.5 | NC_005261.1 | + | 33845 | 0.66 | 0.890003 |
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Target: 5'- -cCCUGGcgcUGGgcgggccgGGAGGGCCCCCCGc-- -3' miRNA: 3'- gaGGGCU---ACC--------UCUCUUGGGGGGUuuu -5' |
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| 23704 | 5' | -55.5 | NC_005261.1 | + | 58759 | 0.67 | 0.852753 |
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Target: 5'- aCUCCCGcgcc-GAGAGCgCCCCCAGc- -3' miRNA: 3'- -GAGGGCuaccuCUCUUG-GGGGGUUuu -5' |
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| 23704 | 5' | -55.5 | NC_005261.1 | + | 25334 | 0.67 | 0.836358 |
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Target: 5'- -cCCCGGcuUGGGGGGGcCCuCCCCAc-- -3' miRNA: 3'- gaGGGCU--ACCUCUCUuGG-GGGGUuuu -5' |
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| 23704 | 5' | -55.5 | NC_005261.1 | + | 11675 | 0.69 | 0.754027 |
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Target: 5'- -cCCCGccGGAGccccuGAACCCCgCCGGAGa -3' miRNA: 3'- gaGGGCuaCCUCu----CUUGGGG-GGUUUU- -5' |
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| 23704 | 5' | -55.5 | NC_005261.1 | + | 8363 | 0.69 | 0.724235 |
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Target: 5'- uUCCaagcggguuuGUGGGGAGGGCCCCCCcAAGc -3' miRNA: 3'- gAGGgc--------UACCUCUCUUGGGGGGuUUU- -5' |
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| 23704 | 5' | -55.5 | NC_005261.1 | + | 74431 | 0.7 | 0.714127 |
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Target: 5'- --gCCGA-GGAGAGcGCCCCCCc--- -3' miRNA: 3'- gagGGCUaCCUCUCuUGGGGGGuuuu -5' |
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| 23704 | 5' | -55.5 | NC_005261.1 | + | 70609 | 0.71 | 0.631445 |
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Target: 5'- -gCCCGA---AGAGGGCCCCCCGGc- -3' miRNA: 3'- gaGGGCUaccUCUCUUGGGGGGUUuu -5' |
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| 23704 | 5' | -55.5 | NC_005261.1 | + | 83045 | 0.73 | 0.508543 |
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Target: 5'- gCUCgCCGcgGGAGGGGGCUCCCUg--- -3' miRNA: 3'- -GAG-GGCuaCCUCUCUUGGGGGGuuuu -5' |
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| 23704 | 5' | -55.5 | NC_005261.1 | + | 829 | 1.06 | 0.003853 |
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Target: 5'- cCUCCCGAUGGAGAGAACCCCCCAAAAa -3' miRNA: 3'- -GAGGGCUACCUCUCUUGGGGGGUUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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