Results 81 - 88 of 88 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23708 | 3' | -57.3 | NC_005261.1 | + | 100112 | 0.66 | 0.834513 |
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Target: 5'- gGCGGCuggugCACGCGGugcuGGCCCGcCUg-- -3' miRNA: 3'- gUGCCG-----GUGCGCCu---UCGGGUaGAaca -5' |
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| 23708 | 3' | -57.3 | NC_005261.1 | + | 123408 | 0.66 | 0.834513 |
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Target: 5'- gCGCGGCCGCGCcGGccgcGCCCGgagCgcgGg -3' miRNA: 3'- -GUGCCGGUGCG-CCuu--CGGGUa--Gaa-Ca -5' |
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| 23708 | 3' | -57.3 | NC_005261.1 | + | 52449 | 0.66 | 0.834513 |
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Target: 5'- gGCGGCCAgggcgcCGCGGAAGCUgG-CgUGg -3' miRNA: 3'- gUGCCGGU------GCGCCUUCGGgUaGaACa -5' |
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| 23708 | 3' | -57.3 | NC_005261.1 | + | 56480 | 0.66 | 0.840281 |
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Target: 5'- aCGCGGCCAgcuccuccuugaggUGCGGGagcaGGCCCAcguUCUc-- -3' miRNA: 3'- -GUGCCGGU--------------GCGCCU----UCGGGU---AGAaca -5' |
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| 23708 | 3' | -57.3 | NC_005261.1 | + | 55094 | 0.66 | 0.841912 |
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Target: 5'- gCACGGCUGCGCGGGgucgugcAGCUCGg----- -3' miRNA: 3'- -GUGCCGGUGCGCCU-------UCGGGUagaaca -5' |
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| 23708 | 3' | -57.3 | NC_005261.1 | + | 58567 | 0.66 | 0.842725 |
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Target: 5'- gCGCGGCgGCGCGGcGGCCag-Cg--- -3' miRNA: 3'- -GUGCCGgUGCGCCuUCGGguaGaaca -5' |
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| 23708 | 3' | -57.3 | NC_005261.1 | + | 48162 | 0.66 | 0.842725 |
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Target: 5'- gCGCGGCgG-GCGGgcGCgCCGUCggGUa -3' miRNA: 3'- -GUGCCGgUgCGCCuuCG-GGUAGaaCA- -5' |
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| 23708 | 3' | -57.3 | NC_005261.1 | + | 124758 | 0.66 | 0.842725 |
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Target: 5'- gUACGGCugCACGCGGAAGCgCGc----- -3' miRNA: 3'- -GUGCCG--GUGCGCCUUCGgGUagaaca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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