Results 81 - 88 of 88 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23708 | 3' | -57.3 | NC_005261.1 | + | 123408 | 0.66 | 0.834513 |
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Target: 5'- gCGCGGCCGCGCcGGccgcGCCCGgagCgcgGg -3' miRNA: 3'- -GUGCCGGUGCG-CCuu--CGGGUa--Gaa-Ca -5' |
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| 23708 | 3' | -57.3 | NC_005261.1 | + | 123858 | 0.67 | 0.772293 |
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Target: 5'- gGCGGUgGCGCGGccGCCCggCg--- -3' miRNA: 3'- gUGCCGgUGCGCCuuCGGGuaGaaca -5' |
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| 23708 | 3' | -57.3 | NC_005261.1 | + | 124027 | 0.69 | 0.632563 |
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Target: 5'- cCGCGGCCggggcgGCGCGGuGGCCCGcaUCg--- -3' miRNA: 3'- -GUGCCGG------UGCGCCuUCGGGU--AGaaca -5' |
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| 23708 | 3' | -57.3 | NC_005261.1 | + | 124758 | 0.66 | 0.842725 |
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Target: 5'- gUACGGCugCACGCGGAAGCgCGc----- -3' miRNA: 3'- -GUGCCG--GUGCGCCUUCGgGUagaaca -5' |
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| 23708 | 3' | -57.3 | NC_005261.1 | + | 125618 | 0.67 | 0.790832 |
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Target: 5'- gGCGGUgGCG-GGggGCUCGUCg--- -3' miRNA: 3'- gUGCCGgUGCgCCuuCGGGUAGaaca -5' |
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| 23708 | 3' | -57.3 | NC_005261.1 | + | 126243 | 0.7 | 0.601803 |
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Target: 5'- gGCGGCCGCGCaGGccccAAGCCCcgCa--- -3' miRNA: 3'- gUGCCGGUGCG-CC----UUCGGGuaGaaca -5' |
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| 23708 | 3' | -57.3 | NC_005261.1 | + | 135804 | 0.7 | 0.6223 |
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Target: 5'- gGCGGCaCGCgGCGGAAGCCgcCGUCg--- -3' miRNA: 3'- gUGCCG-GUG-CGCCUUCGG--GUAGaaca -5' |
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| 23708 | 3' | -57.3 | NC_005261.1 | + | 136912 | 0.69 | 0.653081 |
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Target: 5'- gCGCGGCCGCGCaGAAGgCCAc----- -3' miRNA: 3'- -GUGCCGGUGCGcCUUCgGGUagaaca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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