Results 81 - 88 of 88 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23708 | 3' | -57.3 | NC_005261.1 | + | 15154 | 0.71 | 0.521257 |
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Target: 5'- aGCGGCCgcccagGCGCGGGccccGCCCAUCgcccgUGa -3' miRNA: 3'- gUGCCGG------UGCGCCUu---CGGGUAGa----ACa -5' |
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| 23708 | 3' | -57.3 | NC_005261.1 | + | 13631 | 0.7 | 0.598735 |
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Target: 5'- aGCGGCCGCcuccaggcugaggaGCGGggGCCCuUCc--- -3' miRNA: 3'- gUGCCGGUG--------------CGCCuuCGGGuAGaaca -5' |
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| 23708 | 3' | -57.3 | NC_005261.1 | + | 12312 | 0.67 | 0.790832 |
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Target: 5'- -cCGGCCucgGCGGGcgcGCCCAUCUUc- -3' miRNA: 3'- guGCCGGug-CGCCUu--CGGGUAGAAca -5' |
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| 23708 | 3' | -57.3 | NC_005261.1 | + | 11497 | 0.67 | 0.76283 |
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Target: 5'- gCAgGGCCcuCGgGGAAGCCCAgCUcgGUc -3' miRNA: 3'- -GUgCCGGu-GCgCCUUCGGGUaGAa-CA- -5' |
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| 23708 | 3' | -57.3 | NC_005261.1 | + | 7800 | 0.74 | 0.367691 |
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Target: 5'- gCGCGGCCcCGCGccuGGCCCGUCUUu- -3' miRNA: 3'- -GUGCCGGuGCGCcu-UCGGGUAGAAca -5' |
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| 23708 | 3' | -57.3 | NC_005261.1 | + | 6463 | 0.69 | 0.672512 |
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Target: 5'- -uUGGCgCGCGCGGGAccgcggugcccgcGCCCGUCUa-- -3' miRNA: 3'- guGCCG-GUGCGCCUU-------------CGGGUAGAaca -5' |
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| 23708 | 3' | -57.3 | NC_005261.1 | + | 6215 | 0.67 | 0.79989 |
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Target: 5'- gGCaGCgCGgGCGGggGCCCGcauugCUUGg -3' miRNA: 3'- gUGcCG-GUgCGCCuuCGGGUa----GAACa -5' |
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| 23708 | 3' | -57.3 | NC_005261.1 | + | 4999 | 0.68 | 0.70393 |
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Target: 5'- cCACGGgCGCGCGGcccaaaacaAGGCCCGcUCgcuggUGUc -3' miRNA: 3'- -GUGCCgGUGCGCC---------UUCGGGU-AGa----ACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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