Results 81 - 88 of 88 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23708 | 3' | -57.3 | NC_005261.1 | + | 66613 | 0.67 | 0.798991 |
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Target: 5'- cCGCGGCCcgcucgcagcugaGCGCGGcccGGCCCGUg---- -3' miRNA: 3'- -GUGCCGG-------------UGCGCCu--UCGGGUAgaaca -5' |
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| 23708 | 3' | -57.3 | NC_005261.1 | + | 12312 | 0.67 | 0.790832 |
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Target: 5'- -cCGGCCucgGCGGGcgcGCCCAUCUUc- -3' miRNA: 3'- guGCCGGug-CGCCUu--CGGGUAGAAca -5' |
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| 23708 | 3' | -57.3 | NC_005261.1 | + | 21873 | 0.67 | 0.790832 |
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Target: 5'- cCGCGGCgGCGCG-AGGCCCGc----- -3' miRNA: 3'- -GUGCCGgUGCGCcUUCGGGUagaaca -5' |
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| 23708 | 3' | -57.3 | NC_005261.1 | + | 125618 | 0.67 | 0.790832 |
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Target: 5'- gGCGGUgGCG-GGggGCUCGUCg--- -3' miRNA: 3'- gUGCCGgUGCgCCuuCGGGUAGaaca -5' |
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| 23708 | 3' | -57.3 | NC_005261.1 | + | 94532 | 0.67 | 0.785328 |
|
Target: 5'- aCGCGGCCgcgucguccucggcgGCGCGGcugaucAAGCUCAUCg--- -3' miRNA: 3'- -GUGCCGG---------------UGCGCC------UUCGGGUAGaaca -5' |
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| 23708 | 3' | -57.3 | NC_005261.1 | + | 19333 | 0.67 | 0.78163 |
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Target: 5'- cCGCGGCCGCGCGuGugcuuucguGGCCCGg----- -3' miRNA: 3'- -GUGCCGGUGCGC-Cu--------UCGGGUagaaca -5' |
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| 23708 | 3' | -57.3 | NC_005261.1 | + | 71329 | 0.67 | 0.78163 |
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Target: 5'- gGCgGGCgCGCGCGGggGCUCGcUCg--- -3' miRNA: 3'- gUG-CCG-GUGCGCCuuCGGGU-AGaaca -5' |
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| 23708 | 3' | -57.3 | NC_005261.1 | + | 44866 | 0.68 | 0.743564 |
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Target: 5'- -uUGGCCGCGaGGAAGCCCGc----- -3' miRNA: 3'- guGCCGGUGCgCCUUCGGGUagaaca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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