Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 23711 | 5' | -55.8 | NC_005261.1 | + | 73931 | 0.66 | 0.892035 |
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Target: 5'- aCUGC--GUGUGCCuGCUcCGGcuugcagacgcgGGGCGCg -3' miRNA: 3'- -GACGucUACAUGG-UGA-GCCa-----------CCCGCG- -5' |
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| 23711 | 5' | -55.8 | NC_005261.1 | + | 47413 | 0.66 | 0.88657 |
|
Target: 5'- gUGCAcacGcgGUACUcC-CGGUGGGCGa -3' miRNA: 3'- gACGU---CuaCAUGGuGaGCCACCCGCg -5' |
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| 23711 | 5' | -55.8 | NC_005261.1 | + | 35010 | 0.66 | 0.879534 |
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Target: 5'- cCUGCGG-UGcgGCCGgcguCUCGGUcgcuGGCGCg -3' miRNA: 3'- -GACGUCuACa-UGGU----GAGCCAc---CCGCG- -5' |
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| 23711 | 5' | -55.8 | NC_005261.1 | + | 24126 | 0.66 | 0.879534 |
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Target: 5'- -gGCGGggGUGgggGCUgGGUGGGCGg -3' miRNA: 3'- gaCGUCuaCAUgg-UGAgCCACCCGCg -5' |
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| 23711 | 5' | -55.8 | NC_005261.1 | + | 113873 | 0.66 | 0.908763 |
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Target: 5'- gUGCuccggGUGCCACcuguagcggcgacgCGGcGGGCGCg -3' miRNA: 3'- gACGucua-CAUGGUGa-------------GCCaCCCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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