Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2372 | 3' | -54 | NC_001416.1 | + | 15864 | 0.66 | 0.744452 |
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Target: 5'- cGAUGuUUGCaGACGUaauGGUGCgGGUGAUc -3' miRNA: 3'- -CUGC-AAUGaCUGCA---CCACGgUCGCUG- -5' |
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| 2372 | 3' | -54 | NC_001416.1 | + | 5387 | 1.1 | 0.000906 |
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Target: 5'- uGACGUUACUGACGUGGUGCCAGCGACg -3' miRNA: 3'- -CUGCAAUGACUGCACCACGGUCGCUG- -5' |
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| 2372 | 3' | -54 | NC_001416.1 | + | 5992 | 0.71 | 0.419927 |
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Target: 5'- gGAUGUgcuCUGGCcggaGGcUGCCAGCGACg -3' miRNA: 3'- -CUGCAau-GACUGca--CC-ACGGUCGCUG- -5' |
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| 2372 | 3' | -54 | NC_001416.1 | + | 9226 | 0.7 | 0.469894 |
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Target: 5'- aGGCGcUGCUGGCGUGGUuuaauGaaGGCGAUa -3' miRNA: 3'- -CUGCaAUGACUGCACCA-----CggUCGCUG- -5' |
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| 2372 | 3' | -54 | NC_001416.1 | + | 14593 | 0.68 | 0.588845 |
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Target: 5'- cGACGggGCUGuAUcaGGUGCCguugucAGCGGCa -3' miRNA: 3'- -CUGCaaUGAC-UGcaCCACGG------UCGCUG- -5' |
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| 2372 | 3' | -54 | NC_001416.1 | + | 22174 | 0.67 | 0.645053 |
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Target: 5'- -cCGUcuaUGACGUGGcuuCCGGCGACg -3' miRNA: 3'- cuGCAaugACUGCACCac-GGUCGCUG- -5' |
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| 2372 | 3' | -54 | NC_001416.1 | + | 4539 | 0.66 | 0.711952 |
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Target: 5'- ------cCUGACGgaugcGGUGuCCGGCGACa -3' miRNA: 3'- cugcaauGACUGCa----CCAC-GGUCGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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