Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2372 | 5' | -62.3 | NC_001416.1 | + | 21295 | 0.69 | 0.207444 |
Target: 5'- uGAugGCCAcGgGauCGGCGCAccuGCCGGAa -3' miRNA: 3'- uCUugCGGU-CgC--GCCGCGU---CGGCCUg -5' |
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2372 | 5' | -62.3 | NC_001416.1 | + | 19233 | 0.67 | 0.258754 |
Target: 5'- uGAGaGCCuGCGUGGaCGUuaugugagcgugauGGCCGGACc -3' miRNA: 3'- uCUUgCGGuCGCGCC-GCG--------------UCGGCCUG- -5' |
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2372 | 5' | -62.3 | NC_001416.1 | + | 18503 | 0.67 | 0.260696 |
Target: 5'- --cGCGCCAGCGUGaaaGCAGUgUGGACu -3' miRNA: 3'- ucuUGCGGUCGCGCcg-CGUCG-GCCUG- -5' |
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2372 | 5' | -62.3 | NC_001416.1 | + | 16961 | 0.66 | 0.294832 |
Target: 5'- cGGugGCguGUGCuGGCGguGaacagccagacCCGGACg -3' miRNA: 3'- uCUugCGguCGCG-CCGCguC-----------GGCCUG- -5' |
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2372 | 5' | -62.3 | NC_001416.1 | + | 16873 | 0.71 | 0.15126 |
Target: 5'- uGGAUuUCAGCGuCGGCGCAGaaGGGCu -3' miRNA: 3'- uCUUGcGGUCGC-GCCGCGUCggCCUG- -5' |
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2372 | 5' | -62.3 | NC_001416.1 | + | 14610 | 0.66 | 0.302063 |
Target: 5'- ---gUGCCGuugucaGCGGCaCAGCCGGGCg -3' miRNA: 3'- ucuuGCGGUcg----CGCCGcGUCGGCCUG- -5' |
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2372 | 5' | -62.3 | NC_001416.1 | + | 14158 | 0.68 | 0.247971 |
Target: 5'- cGAuguGCGCCAGCggagucguGCGGCuucgugguaaGCAcGCCGGAg -3' miRNA: 3'- uCU---UGCGGUCG--------CGCCG----------CGU-CGGCCUg -5' |
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2372 | 5' | -62.3 | NC_001416.1 | + | 13459 | 0.68 | 0.229851 |
Target: 5'- cGGAGCagucgGCCAGCGUGGUGCu-CUGGGa -3' miRNA: 3'- -UCUUG-----CGGUCGCGCCGCGucGGCCUg -5' |
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2372 | 5' | -62.3 | NC_001416.1 | + | 12184 | 0.79 | 0.036533 |
Target: 5'- aGGAGCGCCugaacGCGCuGGCGCAGCaggCGGAUa -3' miRNA: 3'- -UCUUGCGGu----CGCG-CCGCGUCG---GCCUG- -5' |
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2372 | 5' | -62.3 | NC_001416.1 | + | 6015 | 0.75 | 0.07621 |
Target: 5'- uAGAACGUCAGCGUGGUGCuGGuCUGGuCa -3' miRNA: 3'- -UCUUGCGGUCGCGCCGCG-UC-GGCCuG- -5' |
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2372 | 5' | -62.3 | NC_001416.1 | + | 5421 | 1.09 | 0.000153 |
Target: 5'- gAGAACGCCAGCGCGGCGCAGCCGGACg -3' miRNA: 3'- -UCUUGCGGUCGCGCCGCGUCGGCCUG- -5' |
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2372 | 5' | -62.3 | NC_001416.1 | + | 4754 | 0.66 | 0.294832 |
Target: 5'- -cAGCGCCcGCGUccGGCugaccaGCGuGCCGGACa -3' miRNA: 3'- ucUUGCGGuCGCG--CCG------CGU-CGGCCUG- -5' |
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2372 | 5' | -62.3 | NC_001416.1 | + | 4693 | 0.67 | 0.260696 |
Target: 5'- cGGACGCgGGCGC--UGCAGCCGuACu -3' miRNA: 3'- uCUUGCGgUCGCGccGCGUCGGCcUG- -5' |
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2372 | 5' | -62.3 | NC_001416.1 | + | 3340 | 0.66 | 0.311667 |
Target: 5'- cGGAugGUCAGCccgaagcgcaucaGCaacccgaacaauaccGGCGaCAGCCGGAa -3' miRNA: 3'- -UCUugCGGUCG-------------CG---------------CCGC-GUCGGCCUg -5' |
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2372 | 5' | -62.3 | NC_001416.1 | + | 3171 | 0.68 | 0.247971 |
Target: 5'- cAGAuaGCGCCAGCuugGGCGaugacugAGCCGGAa -3' miRNA: 3'- -UCU--UGCGGUCGcg-CCGCg------UCGGCCUg -5' |
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2372 | 5' | -62.3 | NC_001416.1 | + | 442 | 0.67 | 0.280775 |
Target: 5'- cGAACGCCAucgacuuaCGCGuGCGCAgGCC-GACg -3' miRNA: 3'- uCUUGCGGUc-------GCGC-CGCGU-CGGcCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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