Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23727 | 3' | -54.3 | NC_005261.1 | + | 133711 | 0.66 | 0.959186 |
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Target: 5'- -----cGACCCCGG-CGCCGUCuACg -3' miRNA: 3'- gcacuaCUGGGGUUaGCGGCAGcUGg -5' |
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| 23727 | 3' | -54.3 | NC_005261.1 | + | 111784 | 0.66 | 0.959186 |
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Target: 5'- cCGgGGUGuGCCCCuggaCGCCuggcucgcGUCGGCCg -3' miRNA: 3'- -GCaCUAC-UGGGGuua-GCGG--------CAGCUGG- -5' |
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| 23727 | 3' | -54.3 | NC_005261.1 | + | 10280 | 0.66 | 0.959186 |
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Target: 5'- -cUGAggacUGgcGCCCCGAcgcgcagcgccUCGCCGUUGACg -3' miRNA: 3'- gcACU----AC--UGGGGUU-----------AGCGGCAGCUGg -5' |
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| 23727 | 3' | -54.3 | NC_005261.1 | + | 51429 | 0.66 | 0.959186 |
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Target: 5'- -cUGcugGGCCCCGGgcgCGCCGUgaUGAUCa -3' miRNA: 3'- gcACua-CUGGGGUUa--GCGGCA--GCUGG- -5' |
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| 23727 | 3' | -54.3 | NC_005261.1 | + | 46164 | 0.66 | 0.959186 |
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Target: 5'- ----cUGcGCCCCAGUCGCCGaUgGGCg -3' miRNA: 3'- gcacuAC-UGGGGUUAGCGGC-AgCUGg -5' |
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| 23727 | 3' | -54.3 | NC_005261.1 | + | 61009 | 0.66 | 0.962708 |
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Target: 5'- -----cGGCUCCGgcAUCGCCGUCGuggggcggcACCg -3' miRNA: 3'- gcacuaCUGGGGU--UAGCGGCAGC---------UGG- -5' |
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| 23727 | 3' | -54.3 | NC_005261.1 | + | 130803 | 0.66 | 0.962708 |
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Target: 5'- gCG-GAcGACgCCCAAUaCGUCGagGGCCa -3' miRNA: 3'- -GCaCUaCUG-GGGUUA-GCGGCagCUGG- -5' |
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| 23727 | 3' | -54.3 | NC_005261.1 | + | 93243 | 0.66 | 0.962708 |
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Target: 5'- -----cGGCCgCC-GUCGCCuUCGACCg -3' miRNA: 3'- gcacuaCUGG-GGuUAGCGGcAGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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