Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23727 | 3' | -54.3 | NC_005261.1 | + | 127961 | 0.71 | 0.769543 |
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Target: 5'- aCGUGGUcgUCCgAAUCGUCGUCGuaGCCg -3' miRNA: 3'- -GCACUAcuGGGgUUAGCGGCAGC--UGG- -5' |
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| 23727 | 3' | -54.3 | NC_005261.1 | + | 34329 | 0.71 | 0.769543 |
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Target: 5'- --cGAggGGCCCCAAgggGCCGgggCGGCCg -3' miRNA: 3'- gcaCUa-CUGGGGUUag-CGGCa--GCUGG- -5' |
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| 23727 | 3' | -54.3 | NC_005261.1 | + | 63804 | 0.71 | 0.760072 |
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Target: 5'- gCGUGAgcgUGAaCCCGA-CGCCGcCGACUg -3' miRNA: 3'- -GCACU---ACUgGGGUUaGCGGCaGCUGG- -5' |
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| 23727 | 3' | -54.3 | NC_005261.1 | + | 16208 | 0.71 | 0.760072 |
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Target: 5'- gCGUGAUGGCCUCGAg-GC--UCGGCCa -3' miRNA: 3'- -GCACUACUGGGGUUagCGgcAGCUGG- -5' |
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| 23727 | 3' | -54.3 | NC_005261.1 | + | 39772 | 0.72 | 0.731016 |
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Target: 5'- --gGAUGGCCUCGaggacGUCGCCGgggggcggcgCGGCCg -3' miRNA: 3'- gcaCUACUGGGGU-----UAGCGGCa---------GCUGG- -5' |
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| 23727 | 3' | -54.3 | NC_005261.1 | + | 106278 | 0.73 | 0.660649 |
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Target: 5'- cCG-GgcGGCCCgugCAAaCGCCGUCGACCu -3' miRNA: 3'- -GCaCuaCUGGG---GUUaGCGGCAGCUGG- -5' |
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| 23727 | 3' | -54.3 | NC_005261.1 | + | 39737 | 0.74 | 0.578972 |
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Target: 5'- gCG-GGUG-CCCCAGUCGCgGaucUCGGCCu -3' miRNA: 3'- -GCaCUACuGGGGUUAGCGgC---AGCUGG- -5' |
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| 23727 | 3' | -54.3 | NC_005261.1 | + | 19889 | 0.77 | 0.425497 |
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Target: 5'- cCGUcGUcGCCCCAGUCGCCGUCGuCg -3' miRNA: 3'- -GCAcUAcUGGGGUUAGCGGCAGCuGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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