Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23727 | 3' | -54.3 | NC_005261.1 | + | 15237 | 0.68 | 0.885449 |
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Target: 5'- gCGuUGA-GGCCCCGcUCGCaGUgGGCCg -3' miRNA: 3'- -GC-ACUaCUGGGGUuAGCGgCAgCUGG- -5' |
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| 23727 | 3' | -54.3 | NC_005261.1 | + | 12129 | 0.68 | 0.898807 |
|
Target: 5'- --gGAUGGCgCCCGc-CGCCGcggCGGCCg -3' miRNA: 3'- gcaCUACUG-GGGUuaGCGGCa--GCUGG- -5' |
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| 23727 | 3' | -54.3 | NC_005261.1 | + | 10517 | 0.68 | 0.892242 |
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Target: 5'- cCGcUGcgGGCCCCGcggcgcgcUCGCUGUCGcCCc -3' miRNA: 3'- -GC-ACuaCUGGGGUu-------AGCGGCAGCuGG- -5' |
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| 23727 | 3' | -54.3 | NC_005261.1 | + | 10280 | 0.66 | 0.959186 |
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Target: 5'- -cUGAggacUGgcGCCCCGAcgcgcagcgccUCGCCGUUGACg -3' miRNA: 3'- gcACU----AC--UGGGGUU-----------AGCGGCAGCUGg -5' |
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| 23727 | 3' | -54.3 | NC_005261.1 | + | 5048 | 0.67 | 0.942835 |
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Target: 5'- -------cCCCCucGUCGUCGUCGGCCc -3' miRNA: 3'- gcacuacuGGGGu-UAGCGGCAGCUGG- -5' |
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| 23727 | 3' | -54.3 | NC_005261.1 | + | 4503 | 0.66 | 0.94727 |
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Target: 5'- --cGcgGGCCCCAGUCGCg--CGcCCg -3' miRNA: 3'- gcaCuaCUGGGGUUAGCGgcaGCuGG- -5' |
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| 23727 | 3' | -54.3 | NC_005261.1 | + | 2498 | 0.66 | 0.955442 |
|
Target: 5'- gCG-GGUGGuCCgCGAgccgCGCCG-CGACCu -3' miRNA: 3'- -GCaCUACU-GGgGUUa---GCGGCaGCUGG- -5' |
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| 23727 | 3' | -54.3 | NC_005261.1 | + | 1211 | 0.68 | 0.90514 |
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Target: 5'- --cGcgGACCCCccgccGAUgcCGCCGUCG-CCg -3' miRNA: 3'- gcaCuaCUGGGG-----UUA--GCGGCAGCuGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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