Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 2373 | 5' | -54.4 | NC_001416.1 | + | 5573 | 1.11 | 0.000696 |
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Target: 5'- cGUGAAAACGGCCCGCCGCAUUCUGGCc -3' miRNA: 3'- -CACUUUUGCCGGGCGGCGUAAGACCG- -5' |
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| 2373 | 5' | -54.4 | NC_001416.1 | + | 472 | 0.7 | 0.48791 |
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Target: 5'- cUGGAGAucugccucgcUGGCCUGCCGCAgUUCUucaaccucccGGCg -3' miRNA: 3'- cACUUUU----------GCCGGGCGGCGU-AAGA----------CCG- -5' |
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| 2373 | 5' | -54.4 | NC_001416.1 | + | 36743 | 0.69 | 0.509036 |
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Target: 5'- uGUGGu--CGGCCCGaaaacaGUUCUGGCa -3' miRNA: 3'- -CACUuuuGCCGGGCggcg--UAAGACCG- -5' |
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| 2373 | 5' | -54.4 | NC_001416.1 | + | 17422 | 0.68 | 0.619139 |
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Target: 5'- aGUGAcuucuGCGGUCagguGCUGCAccgCUGGCg -3' miRNA: 3'- -CACUuu---UGCCGGg---CGGCGUaa-GACCG- -5' |
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| 2373 | 5' | -54.4 | NC_001416.1 | + | 8017 | 0.67 | 0.641593 |
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Target: 5'- -aGcAAAACGGCggugccuggugCCGCCGCAa--UGGCc -3' miRNA: 3'- caC-UUUUGCCG-----------GGCGGCGUaagACCG- -5' |
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| 2373 | 5' | -54.4 | NC_001416.1 | + | 47553 | 0.67 | 0.652812 |
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Target: 5'- -aGu--GCGGuCCUGCUgGCAUUCUGGa -3' miRNA: 3'- caCuuuUGCC-GGGCGG-CGUAAGACCg -5' |
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| 2373 | 5' | -54.4 | NC_001416.1 | + | 5493 | 0.66 | 0.719313 |
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Target: 5'- ------cCGGCUgCGCCGCg--CUGGCg -3' miRNA: 3'- cacuuuuGCCGG-GCGGCGuaaGACCG- -5' |
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| 2373 | 5' | -54.4 | NC_001416.1 | + | 3586 | 0.66 | 0.707287 |
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Target: 5'- cGUGAAugaacgaGGCGcGCCCGCCGgGUaacUCacggGGUa -3' miRNA: 3'- -CACUU-------UUGC-CGGGCGGCgUA---AGa---CCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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