Results 81 - 88 of 88 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23732 | 5' | -63.4 | NC_005261.1 | + | 4837 | 0.66 | 0.548513 |
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Target: 5'- aGGCGGCgGCgUCCgCGCCGcagccucaAGGgcGg- -3' miRNA: 3'- -CCGCCGgCG-AGGgGCGGC--------UCCauCaa -5' |
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| 23732 | 5' | -63.4 | NC_005261.1 | + | 77337 | 0.66 | 0.548513 |
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Target: 5'- cGGC-GCCGCcCCCCGCCauGAGcGcAGUg -3' miRNA: 3'- -CCGcCGGCGaGGGGCGG--CUC-CaUCAa -5' |
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| 23732 | 5' | -63.4 | NC_005261.1 | + | 16313 | 0.66 | 0.548513 |
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Target: 5'- cGGCGGCCGggccCUCCCCcucGCCGccGGUc--- -3' miRNA: 3'- -CCGCCGGC----GAGGGG---CGGCu-CCAucaa -5' |
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| 23732 | 5' | -63.4 | NC_005261.1 | + | 136614 | 0.66 | 0.548513 |
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Target: 5'- aGCGGCgCGCg-CgCGCCGAGGgcGg- -3' miRNA: 3'- cCGCCG-GCGagGgGCGGCUCCauCaa -5' |
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| 23732 | 5' | -63.4 | NC_005261.1 | + | 70537 | 0.66 | 0.558142 |
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Target: 5'- cGGCGGCgccCGCgccccaCCCGCCGcAGGgcGc- -3' miRNA: 3'- -CCGCCG---GCGag----GGGCGGC-UCCauCaa -5' |
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| 23732 | 5' | -63.4 | NC_005261.1 | + | 87503 | 0.66 | 0.567819 |
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Target: 5'- uGGCGcGCaggCGCUCgCCGCCGuGGaAGa- -3' miRNA: 3'- -CCGC-CG---GCGAGgGGCGGCuCCaUCaa -5' |
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| 23732 | 5' | -63.4 | NC_005261.1 | + | 91172 | 0.66 | 0.567819 |
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Target: 5'- gGGCGGCaGCU-CCCGCCGGGc----- -3' miRNA: 3'- -CCGCCGgCGAgGGGCGGCUCcaucaa -5' |
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| 23732 | 5' | -63.4 | NC_005261.1 | + | 116836 | 0.66 | 0.567819 |
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Target: 5'- cGUGGCCG-UCCCCGCCGc-GaAGUUc -3' miRNA: 3'- cCGCCGGCgAGGGGCGGCucCaUCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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