Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23736 | 3' | -56.8 | NC_005261.1 | + | 36828 | 0.66 | 0.854275 |
Target: 5'- cGGUAUG-CgGUGCCGGcGC-CCGGGGg -3' miRNA: 3'- cUCGUACaGgUACGGCU-CGaGGUCCU- -5' |
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23736 | 3' | -56.8 | NC_005261.1 | + | 108164 | 0.66 | 0.852688 |
Target: 5'- cAGCGcGUCCGccagggcgcgcgGCCGcGGCUCCGGGc -3' miRNA: 3'- cUCGUaCAGGUa-----------CGGC-UCGAGGUCCu -5' |
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23736 | 3' | -56.8 | NC_005261.1 | + | 47105 | 0.66 | 0.846258 |
Target: 5'- aGAGCccGUCCGUGaggaCGuGCgcgUCCAGGu -3' miRNA: 3'- -CUCGuaCAGGUACg---GCuCG---AGGUCCu -5' |
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23736 | 3' | -56.8 | NC_005261.1 | + | 58012 | 0.66 | 0.845445 |
Target: 5'- aGGaCGUGgaaGUGCCGcggcggcAGCUCCAGGGa -3' miRNA: 3'- cUC-GUACaggUACGGC-------UCGAGGUCCU- -5' |
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23736 | 3' | -56.8 | NC_005261.1 | + | 121448 | 0.66 | 0.829631 |
Target: 5'- cGGGCAUGgggCCGucgaUGgCGAGCUCCucGGc -3' miRNA: 3'- -CUCGUACa--GGU----ACgGCUCGAGGu-CCu -5' |
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23736 | 3' | -56.8 | NC_005261.1 | + | 99541 | 0.67 | 0.803327 |
Target: 5'- aGGGCGcGUCCAgGCgGcGCgCCAGGAa -3' miRNA: 3'- -CUCGUaCAGGUaCGgCuCGaGGUCCU- -5' |
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23736 | 3' | -56.8 | NC_005261.1 | + | 121962 | 0.67 | 0.79423 |
Target: 5'- cAGCucg-CCGUGCCGcGCUCCAGc- -3' miRNA: 3'- cUCGuacaGGUACGGCuCGAGGUCcu -5' |
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23736 | 3' | -56.8 | NC_005261.1 | + | 15645 | 0.67 | 0.756428 |
Target: 5'- uAGCGccgCCcgGCCGGgagcugguGCUCCAGGAa -3' miRNA: 3'- cUCGUacaGGuaCGGCU--------CGAGGUCCU- -5' |
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23736 | 3' | -56.8 | NC_005261.1 | + | 40688 | 0.67 | 0.755457 |
Target: 5'- gGAGCcgGcgCCG-GCCGGGCUCCuccgaaagcagcgAGGGg -3' miRNA: 3'- -CUCGuaCa-GGUaCGGCUCGAGG-------------UCCU- -5' |
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23736 | 3' | -56.8 | NC_005261.1 | + | 24526 | 0.68 | 0.723856 |
Target: 5'- uGAGCAaggUGUCCccgcccacguaccugGCCGGGCggcgCCAGGu -3' miRNA: 3'- -CUCGU---ACAGGua-------------CGGCUCGa---GGUCCu -5' |
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23736 | 3' | -56.8 | NC_005261.1 | + | 45188 | 0.73 | 0.454499 |
Target: 5'- cGAGUGUGaggCCAUGCCG-GCcuUCCGGGGc -3' miRNA: 3'- -CUCGUACa--GGUACGGCuCG--AGGUCCU- -5' |
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23736 | 3' | -56.8 | NC_005261.1 | + | 95347 | 0.75 | 0.342979 |
Target: 5'- cGGGCGUGUCCGUGCUc--UUCCAGGGg -3' miRNA: 3'- -CUCGUACAGGUACGGcucGAGGUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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