Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
23737 | 3' | -53.8 | NC_005261.1 | + | 78537 | 0.66 | 0.949601 |
Target: 5'- cGGCgaggGGAAGGGGAgCCgCGAUCCg- -3' miRNA: 3'- -UCGaga-CCUUCCUUUaGG-GCUAGGgc -5' |
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23737 | 3' | -53.8 | NC_005261.1 | + | 32768 | 0.67 | 0.919534 |
Target: 5'- gGGCUCggaGGGAGGAGAgggaggCCCGG-CUgGg -3' miRNA: 3'- -UCGAGa--CCUUCCUUUa-----GGGCUaGGgC- -5' |
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23737 | 3' | -53.8 | NC_005261.1 | + | 30898 | 0.68 | 0.901157 |
Target: 5'- gAGCUCgGGcuGGGGccgCCCGGccccUCCCGg -3' miRNA: 3'- -UCGAGaCCuuCCUUua-GGGCU----AGGGC- -5' |
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23737 | 3' | -53.8 | NC_005261.1 | + | 25471 | 0.71 | 0.749547 |
Target: 5'- gGGCUUgagugGGAAGGGGAUCCCccauaucGAUCgCGc -3' miRNA: 3'- -UCGAGa----CCUUCCUUUAGGG-------CUAGgGC- -5' |
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23737 | 3' | -53.8 | NC_005261.1 | + | 128705 | 0.71 | 0.740648 |
Target: 5'- gAGCUC-GGGAGGcGcgCCCGggCCCc -3' miRNA: 3'- -UCGAGaCCUUCCuUuaGGGCuaGGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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