miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
2374 3' -56.6 NC_001416.1 + 40472 0.66 0.619831
Target:  5'- aGUGUCGCCagagUGCCGcGAUGAaacGGGUa-- -3'
miRNA:   3'- aCGCAGCGG----ACGGC-CUACU---UCUAggc -5'
2374 3' -56.6 NC_001416.1 + 3573 0.68 0.51083
Target:  5'- gGCG-CGCCcGCCGGGUaacucacGggGuAUCCa -3'
miRNA:   3'- aCGCaGCGGaCGGCCUA-------CuuC-UAGGc -5'
2374 3' -56.6 NC_001416.1 + 19301 0.68 0.458771
Target:  5'- gUGCGUaCGCCaugGCCGGAgUGGcucacagucgguGGUCCGg -3'
miRNA:   3'- -ACGCA-GCGGa--CGGCCU-ACUu-----------CUAGGC- -5'
2374 3' -56.6 NC_001416.1 + 39948 0.68 0.460761
Target:  5'- aGCGagGCCUGuauCCGGAUGcGGAgucuuaUCCGu -3'
miRNA:   3'- aCGCagCGGAC---GGCCUACuUCU------AGGC- -5'
2374 3' -56.6 NC_001416.1 + 13178 0.71 0.342091
Target:  5'- aGCGagCGCCUGCCGGGcUGAAug-CCa -3'
miRNA:   3'- aCGCa-GCGGACGGCCU-ACUUcuaGGc -5'
2374 3' -56.6 NC_001416.1 + 6396 1.08 0.000785
Target:  5'- cUGCGUCGCCUGCCGGAUGAAGAUCCGc -3'
miRNA:   3'- -ACGCAGCGGACGGCCUACUUCUAGGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.