Results 21 - 33 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23744 | 3' | -53.5 | NC_005261.1 | + | 19573 | 0.67 | 0.919885 |
Target: 5'- cGGgcGGuuGAUUcuccagAGGUGCGUGUGCAGg -3' miRNA: 3'- cUCaaCCu-UUAG------UCCACGCGCGCGUC- -5' |
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23744 | 3' | -53.5 | NC_005261.1 | + | 13290 | 0.67 | 0.919885 |
Target: 5'- -uGgcGGGGcgCAGGUGUGCGaCGgGGg -3' miRNA: 3'- cuCaaCCUUuaGUCCACGCGC-GCgUC- -5' |
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23744 | 3' | -53.5 | NC_005261.1 | + | 112847 | 0.67 | 0.913943 |
Target: 5'- ----cGGAGAUC--GUGCaGCGCGCGGa -3' miRNA: 3'- cucaaCCUUUAGucCACG-CGCGCGUC- -5' |
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23744 | 3' | -53.5 | NC_005261.1 | + | 67107 | 0.67 | 0.907746 |
Target: 5'- ----cGGug--CGGGcgGCGCGCGCAGu -3' miRNA: 3'- cucaaCCuuuaGUCCa-CGCGCGCGUC- -5' |
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23744 | 3' | -53.5 | NC_005261.1 | + | 105358 | 0.67 | 0.907746 |
Target: 5'- uGAGgcaGGccguGA-CAGGcgGCGCGCGCGGg -3' miRNA: 3'- -CUCaa-CCu---UUaGUCCa-CGCGCGCGUC- -5' |
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23744 | 3' | -53.5 | NC_005261.1 | + | 32902 | 0.66 | 0.954369 |
Target: 5'- ----cGGGAGggaAGGgGCGCGCGUGGg -3' miRNA: 3'- cucaaCCUUUag-UCCaCGCGCGCGUC- -5' |
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23744 | 3' | -53.5 | NC_005261.1 | + | 15049 | 0.66 | 0.954369 |
Target: 5'- -cGggGGggGUCGGG-GuCGCG-GCGGg -3' miRNA: 3'- cuCaaCCuuUAGUCCaC-GCGCgCGUC- -5' |
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23744 | 3' | -53.5 | NC_005261.1 | + | 125377 | 0.66 | 0.950196 |
Target: 5'- aGGGgcgcUGGAA--CGGGUGCGUGCGg-- -3' miRNA: 3'- -CUCa---ACCUUuaGUCCACGCGCGCguc -5' |
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23744 | 3' | -53.5 | NC_005261.1 | + | 96850 | 0.66 | 0.950196 |
Target: 5'- gGGGUcgacgGGggGcUCGGGggGCGCGgGUAGc -3' miRNA: 3'- -CUCAa----CCuuU-AGUCCa-CGCGCgCGUC- -5' |
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23744 | 3' | -53.5 | NC_005261.1 | + | 105126 | 0.66 | 0.945776 |
Target: 5'- ----cGGGcg-CGGGccgccgGCGCGCGCAGg -3' miRNA: 3'- cucaaCCUuuaGUCCa-----CGCGCGCGUC- -5' |
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23744 | 3' | -53.5 | NC_005261.1 | + | 17449 | 0.66 | 0.945776 |
Target: 5'- gGGGUUGGggGUaGGGgaaacUGCGgGCAGa -3' miRNA: 3'- -CUCAACCuuUAgUCCac---GCGCgCGUC- -5' |
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23744 | 3' | -53.5 | NC_005261.1 | + | 97572 | 0.66 | 0.941106 |
Target: 5'- gGGGUUGGc--UCGGuG-GCGCGgGCGGa -3' miRNA: 3'- -CUCAACCuuuAGUC-CaCGCGCgCGUC- -5' |
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23744 | 3' | -53.5 | NC_005261.1 | + | 80652 | 0.66 | 0.954369 |
Target: 5'- cGAGUagcUGGugugcUCGcGGUGCGCGCcgucGCGGa -3' miRNA: 3'- -CUCA---ACCuuu--AGU-CCACGCGCG----CGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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