Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23749 | 5' | -56.4 | NC_005261.1 | + | 68596 | 0.66 | 0.922997 |
Target: 5'- gCCGcgGgagCgGGGCCgaCGG-CGUGGGGGa -3' miRNA: 3'- -GGCa-Caa-GaCCUGG--GCCuGCAUCCCC- -5' |
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23749 | 5' | -56.4 | NC_005261.1 | + | 2551 | 0.66 | 0.922997 |
Target: 5'- gCCGcgGcgCUGGGCgCGGGCGUGuGGu -3' miRNA: 3'- -GGCa-CaaGACCUGgGCCUGCAUcCCc -5' |
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23749 | 5' | -56.4 | NC_005261.1 | + | 33694 | 0.66 | 0.917507 |
Target: 5'- cCCGgggUCgcgcgGGGCCCgcugcacgagGGGCGgUGGGGGc -3' miRNA: 3'- -GGCacaAGa----CCUGGG----------CCUGC-AUCCCC- -5' |
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23749 | 5' | -56.4 | NC_005261.1 | + | 32919 | 0.66 | 0.911788 |
Target: 5'- gCGUGgg--GGGCggCGGGCGgggGGGGGg -3' miRNA: 3'- gGCACaagaCCUGg-GCCUGCa--UCCCC- -5' |
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23749 | 5' | -56.4 | NC_005261.1 | + | 16159 | 0.66 | 0.911788 |
Target: 5'- aCCGgcagGgcaguaggGGGCgCGGGCGUGGcGGGg -3' miRNA: 3'- -GGCa---Caaga----CCUGgGCCUGCAUC-CCC- -5' |
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23749 | 5' | -56.4 | NC_005261.1 | + | 97358 | 0.67 | 0.893282 |
Target: 5'- aCCGUGgggUCUacGGCCCGGGCGUGu--- -3' miRNA: 3'- -GGCACa--AGAc-CUGGGCCUGCAUcccc -5' |
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23749 | 5' | -56.4 | NC_005261.1 | + | 86521 | 0.67 | 0.893282 |
Target: 5'- gCCGUccuugUCgcagcgcgGGuuCCCGGccACGUAGGGGg -3' miRNA: 3'- -GGCAca---AGa-------CCu-GGGCC--UGCAUCCCC- -5' |
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23749 | 5' | -56.4 | NC_005261.1 | + | 22052 | 0.67 | 0.879845 |
Target: 5'- uCCGgcgggGUUCagGGGCuCCGG-CGgggcucgAGGGGg -3' miRNA: 3'- -GGCa----CAAGa-CCUG-GGCCuGCa------UCCCC- -5' |
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23749 | 5' | -56.4 | NC_005261.1 | + | 15930 | 0.67 | 0.865563 |
Target: 5'- gCCG-GggCcgGGGCCCGGGCaGgagccGGGGGc -3' miRNA: 3'- -GGCaCaaGa-CCUGGGCCUG-Ca----UCCCC- -5' |
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23749 | 5' | -56.4 | NC_005261.1 | + | 98427 | 0.67 | 0.865563 |
Target: 5'- gCCGUcccCUgcGGACCCGGGCcgGUAGGcGGc -3' miRNA: 3'- -GGCAcaaGA--CCUGGGCCUG--CAUCC-CC- -5' |
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23749 | 5' | -56.4 | NC_005261.1 | + | 133519 | 0.67 | 0.86112 |
Target: 5'- aCCGUGUUCaccgUGGACCUcucgGGGCGcgcagcgcagcaugcUgcucGGGGGg -3' miRNA: 3'- -GGCACAAG----ACCUGGG----CCUGC---------------A----UCCCC- -5' |
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23749 | 5' | -56.4 | NC_005261.1 | + | 68248 | 0.67 | 0.86112 |
Target: 5'- gCGUGUUCgUGGacuGCCUGGcgcgccugcgcccgcGCGgcGGGGc -3' miRNA: 3'- gGCACAAG-ACC---UGGGCC---------------UGCauCCCC- -5' |
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23749 | 5' | -56.4 | NC_005261.1 | + | 30792 | 0.68 | 0.850477 |
Target: 5'- gCgGUGgaggugCUGGGgCCGGAgGagcUGGGGGg -3' miRNA: 3'- -GgCACaa----GACCUgGGCCUgC---AUCCCC- -5' |
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23749 | 5' | -56.4 | NC_005261.1 | + | 98053 | 0.68 | 0.842647 |
Target: 5'- gCCGcGggCggcgGGACCgCGGGCGgcGGGu -3' miRNA: 3'- -GGCaCaaGa---CCUGG-GCCUGCauCCCc -5' |
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23749 | 5' | -56.4 | NC_005261.1 | + | 83857 | 0.68 | 0.842647 |
Target: 5'- cCCGcUGggCUGGGCcgggCCGGACGccGGGu -3' miRNA: 3'- -GGC-ACaaGACCUG----GGCCUGCauCCCc -5' |
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23749 | 5' | -56.4 | NC_005261.1 | + | 111068 | 0.7 | 0.70747 |
Target: 5'- cCCGUcGUcaggaUCgaGGGCCUGG-UGUGGGGGg -3' miRNA: 3'- -GGCA-CA-----AGa-CCUGGGCCuGCAUCCCC- -5' |
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23749 | 5' | -56.4 | NC_005261.1 | + | 71620 | 0.71 | 0.657927 |
Target: 5'- cCCGgGcgC-GGGCCCGGGCGgcGGGa -3' miRNA: 3'- -GGCaCaaGaCCUGGGCCUGCauCCCc -5' |
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23749 | 5' | -56.4 | NC_005261.1 | + | 29440 | 0.72 | 0.627898 |
Target: 5'- gCCGUGUUC--GugCCGGAgaUGGGGGa -3' miRNA: 3'- -GGCACAAGacCugGGCCUgcAUCCCC- -5' |
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23749 | 5' | -56.4 | NC_005261.1 | + | 128786 | 0.73 | 0.587934 |
Target: 5'- gCCGUugUCUGGgcGCCCGGgcccGCGgcGGGGc -3' miRNA: 3'- -GGCAcaAGACC--UGGGCC----UGCauCCCC- -5' |
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23749 | 5' | -56.4 | NC_005261.1 | + | 36914 | 0.73 | 0.558258 |
Target: 5'- cCCGcg--CUGGGCCgGGACGUAcuccGGGGc -3' miRNA: 3'- -GGCacaaGACCUGGgCCUGCAU----CCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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