miRNA: 3'- gaCUGCGACCa--C-CUGCa-CGUGuuCGU- -5'
miRNA: 3'- gacUGCGACCa---CCUGCA-------------CGUGuUCGU- -5'
miRNA: 3'- gaCUGCGACCACcUGcACGUGU--UCGU- -5'
miRNA: 3'- gaCUGCGa-CCACCUG-CaCGuGUUCGU- -5'
miRNA: 3'- gACUGCGa--CCAcCUGCACGu---GUUCGu -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23751 | 3' | -55.2 | NC_005261.1 | + | 75573 | 0.66 | 0.902228 |
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Target: 5'- -gGGCGCUGGgcgGcGGCGcgGCGCucGCGc -3' miRNA: 3'- gaCUGCGACCa--C-CUGCa-CGUGuuCGU- -5' |
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| 23751 | 3' | -55.2 | NC_005261.1 | + | 36916 | 0.66 | 0.907932 |
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Target: 5'- --cGCGCUGGgccgGGACGUacuccggggcccuGCGCuGGCGc -3' miRNA: 3'- gacUGCGACCa---CCUGCA-------------CGUGuUCGU- -5' |
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| 23751 | 3' | -55.2 | NC_005261.1 | + | 69489 | 0.66 | 0.914635 |
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Target: 5'- -cGAUGCUGGUGcACcUGUACGccGGCGg -3' miRNA: 3'- gaCUGCGACCACcUGcACGUGU--UCGU- -5' |
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| 23751 | 3' | -55.2 | NC_005261.1 | + | 114328 | 0.66 | 0.914635 |
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Target: 5'- -cGGCGCaaGGUGGACuG-GCuCGAGCGg -3' miRNA: 3'- gaCUGCGa-CCACCUG-CaCGuGUUCGU- -5' |
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| 23751 | 3' | -55.2 | NC_005261.1 | + | 43647 | 0.66 | 0.914635 |
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Target: 5'- gUGGCGCggcGGU-GACGUGCucuuCGAGUc -3' miRNA: 3'- gACUGCGa--CCAcCUGCACGu---GUUCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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