miRNA: 3'- gaCUGCGaCCACC--UGCaCGUGUUCGU- -5'
miRNA: 3'- -GACUGCGACCa-------CCUGCAC-GugUUCGU- -5'
miRNA: 3'- gaCUGCGaCCAC-CU---GCACGUGUUCGU- -5'
miRNA: 3'- gaCUGCGACC--AC-CUGCACGUGU-UCGU- -5'
miRNA: 3'- gaCUGCGACCACCUG---CACGUGUuCGU- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23751 | 3' | -55.2 | NC_005261.1 | + | 73225 | 0.73 | 0.526541 |
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Target: 5'- -gGGCGC-GGUGGccGCGaGCGCGAGCAg -3' miRNA: 3'- gaCUGCGaCCACC--UGCaCGUGUUCGU- -5' |
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| 23751 | 3' | -55.2 | NC_005261.1 | + | 14945 | 0.76 | 0.395076 |
|
Target: 5'- gCUGGCGCUGGaagcgagcGGGCGUGcCugGGGCGa -3' miRNA: 3'- -GACUGCGACCa-------CCUGCAC-GugUUCGU- -5' |
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| 23751 | 3' | -55.2 | NC_005261.1 | + | 95673 | 0.76 | 0.369758 |
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Target: 5'- -gGGCGCgGGUGuGAgcaCGUGCACGAGCGc -3' miRNA: 3'- gaCUGCGaCCAC-CU---GCACGUGUUCGU- -5' |
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| 23751 | 3' | -55.2 | NC_005261.1 | + | 73776 | 0.76 | 0.369758 |
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Target: 5'- -aGGCGCUGGccUGcGACGUGCGCGcGGCGg -3' miRNA: 3'- gaCUGCGACC--AC-CUGCACGUGU-UCGU- -5' |
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| 23751 | 3' | -55.2 | NC_005261.1 | + | 29483 | 0.79 | 0.260423 |
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Target: 5'- -cGGCGCUGGUGGACcugGUGUACGcGCGc -3' miRNA: 3'- gaCUGCGACCACCUG---CACGUGUuCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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