miRNA: 3'- gaCUGC-GACCa---CCUGC---ACGUGUUCGU- -5'
miRNA: 3'- -GACUGCGACCa-------CCUGCAC-GugUUCGU- -5'
miRNA: 3'- gaCugCGaCCACCUGCa---CGUGU---UCGU- -5'
miRNA: 3'- gaCUGCGACCaCC--UGCaCGUGuUCGU- -5'
miRNA: 3'- gaCUGCGACCa---CCUGCACGU--------GUUCGU- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23751 | 3' | -55.2 | NC_005261.1 | + | 27243 | 0.69 | 0.752282 |
|
Target: 5'- -aGGCGcCUGGggugGGGCGgggUGUGCGGGCAg -3' miRNA: 3'- gaCUGC-GACCa---CCUGC---ACGUGUUCGU- -5' |
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| 23751 | 3' | -55.2 | NC_005261.1 | + | 14945 | 0.76 | 0.395076 |
|
Target: 5'- gCUGGCGCUGGaagcgagcGGGCGUGcCugGGGCGa -3' miRNA: 3'- -GACUGCGACCa-------CCUGCAC-GugUUCGU- -5' |
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| 23751 | 3' | -55.2 | NC_005261.1 | + | 6099 | 0.68 | 0.826384 |
|
Target: 5'- -gGugGC-GGUGGAUGaaagGCGCAcacGGCAa -3' miRNA: 3'- gaCugCGaCCACCUGCa---CGUGU---UCGU- -5' |
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| 23751 | 3' | -55.2 | NC_005261.1 | + | 3839 | 0.67 | 0.874536 |
|
Target: 5'- -gGGCGCUGGcGGcaGCG-GCGCcGGCGc -3' miRNA: 3'- gaCUGCGACCaCC--UGCaCGUGuUCGU- -5' |
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| 23751 | 3' | -55.2 | NC_005261.1 | + | 2555 | 0.7 | 0.722417 |
|
Target: 5'- -cGGCGCUGGgcgcGGGCGUGUGguacucccCGGGCGg -3' miRNA: 3'- gaCUGCGACCa---CCUGCACGU--------GUUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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