Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23757 | 5' | -56 | NC_005261.1 | + | 18663 | 0.66 | 0.903127 |
Target: 5'- uCGGgcgGGCCCcagcggGCGAAGgggccCCAGGcUGUUg -3' miRNA: 3'- -GCCa--CCGGG------CGCUUCa----GGUUCuACAG- -5' |
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23757 | 5' | -56 | NC_005261.1 | + | 29428 | 0.66 | 0.890003 |
Target: 5'- gCGG-GGCCCGCGGccguGUUCGugccggAGAUGg- -3' miRNA: 3'- -GCCaCCGGGCGCUu---CAGGU------UCUACag -5' |
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23757 | 5' | -56 | NC_005261.1 | + | 88718 | 0.66 | 0.890003 |
Target: 5'- --cUGGCCUGCGggGUgCGcGAUGa- -3' miRNA: 3'- gccACCGGGCGCuuCAgGUuCUACag -5' |
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23757 | 5' | -56 | NC_005261.1 | + | 23929 | 0.66 | 0.883095 |
Target: 5'- gGGUGGgcucacCUCGCGGcgGGUUCGGGGUcGUCg -3' miRNA: 3'- gCCACC------GGGCGCU--UCAGGUUCUA-CAG- -5' |
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23757 | 5' | -56 | NC_005261.1 | + | 18222 | 0.66 | 0.875962 |
Target: 5'- cCGGUccaGCCCGCaGggGUauuuguGGAUGUCg -3' miRNA: 3'- -GCCAc--CGGGCG-CuuCAggu---UCUACAG- -5' |
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23757 | 5' | -56 | NC_005261.1 | + | 34285 | 0.66 | 0.875962 |
Target: 5'- aCGGcGGCCgGCGggGacgCCGGGAg--- -3' miRNA: 3'- -GCCaCCGGgCGCuuCa--GGUUCUacag -5' |
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23757 | 5' | -56 | NC_005261.1 | + | 12747 | 0.67 | 0.86104 |
Target: 5'- uGGgcgGGCCCgGCGAGGUCguAGgcGg- -3' miRNA: 3'- gCCa--CCGGG-CGCUUCAGguUCuaCag -5' |
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23757 | 5' | -56 | NC_005261.1 | + | 54384 | 0.67 | 0.86104 |
Target: 5'- uCGGgGcGCCCGCGAAGgCCGugccgguuaaGGAaGUCg -3' miRNA: 3'- -GCCaC-CGGGCGCUUCaGGU----------UCUaCAG- -5' |
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23757 | 5' | -56 | NC_005261.1 | + | 13137 | 0.67 | 0.848499 |
Target: 5'- gCGuGUGGUCgGUGAcggcgcgcaccaggcGGUCCGAGGUGa- -3' miRNA: 3'- -GC-CACCGGgCGCU---------------UCAGGUUCUACag -5' |
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23757 | 5' | -56 | NC_005261.1 | + | 64510 | 0.67 | 0.837108 |
Target: 5'- uCGG-GGCCCGCGGGcggcuccucGUCgGGGGgcagGUCc -3' miRNA: 3'- -GCCaCCGGGCGCUU---------CAGgUUCUa---CAG- -5' |
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23757 | 5' | -56 | NC_005261.1 | + | 23729 | 0.67 | 0.828743 |
Target: 5'- gGGUGGCCgCGcCGAGgcgcGUCCGGGAg--- -3' miRNA: 3'- gCCACCGG-GC-GCUU----CAGGUUCUacag -5' |
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23757 | 5' | -56 | NC_005261.1 | + | 57417 | 0.67 | 0.828743 |
Target: 5'- gCGGccGGCgCCGCG-AGcCCAGGAUG-Cg -3' miRNA: 3'- -GCCa-CCG-GGCGCuUCaGGUUCUACaG- -5' |
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23757 | 5' | -56 | NC_005261.1 | + | 75933 | 0.68 | 0.81148 |
Target: 5'- -cGUGGCCCGCGuguacGAGgccugccCCGAGGUGg- -3' miRNA: 3'- gcCACCGGGCGC-----UUCa------GGUUCUACag -5' |
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23757 | 5' | -56 | NC_005261.1 | + | 118902 | 0.68 | 0.793559 |
Target: 5'- uGGUGGCCUGCGucugGGUCCGGc----- -3' miRNA: 3'- gCCACCGGGCGCu---UCAGGUUcuacag -5' |
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23757 | 5' | -56 | NC_005261.1 | + | 123284 | 0.68 | 0.784373 |
Target: 5'- gGGUGGUCCGCcagcgccUCCGcGAUGUCc -3' miRNA: 3'- gCCACCGGGCGcuuc---AGGUuCUACAG- -5' |
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23757 | 5' | -56 | NC_005261.1 | + | 1603 | 0.69 | 0.750223 |
Target: 5'- gCGGUGGCuggccgccucgcccuCCGCGAAGUCCGGc----- -3' miRNA: 3'- -GCCACCG---------------GGCGCUUCAGGUUcuacag -5' |
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23757 | 5' | -56 | NC_005261.1 | + | 33494 | 0.69 | 0.746337 |
Target: 5'- gCGG-GGCCCGCGAGcaCCAGGGg--- -3' miRNA: 3'- -GCCaCCGGGCGCUUcaGGUUCUacag -5' |
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23757 | 5' | -56 | NC_005261.1 | + | 46937 | 0.69 | 0.746337 |
Target: 5'- uGGUgcucacgcGGCCCGCGGgccAGUCCGcGccGUCg -3' miRNA: 3'- gCCA--------CCGGGCGCU---UCAGGUuCuaCAG- -5' |
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23757 | 5' | -56 | NC_005261.1 | + | 12907 | 0.7 | 0.675214 |
Target: 5'- uGGUguacaccGGCCCGaaGggGUUgAGGAUGUCg -3' miRNA: 3'- gCCA-------CCGGGCg-CuuCAGgUUCUACAG- -5' |
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23757 | 5' | -56 | NC_005261.1 | + | 24186 | 0.7 | 0.666003 |
Target: 5'- gGGUcGUCgGCGAGGcUCCAGGAUcGUCg -3' miRNA: 3'- gCCAcCGGgCGCUUC-AGGUUCUA-CAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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