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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2376 | 5' | -58.8 | NC_001416.1 | + | 10873 | 1.09 | 0.000389 |
Target: 5'- uGCCGGUGCGAUCACCCUGCCGAUGGUg -3' miRNA: 3'- -CGGCCACGCUAGUGGGACGGCUACCA- -5' |
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2376 | 5' | -58.8 | NC_001416.1 | + | 42429 | 0.67 | 0.458677 |
Target: 5'- uGCCGGgaaugGUu-UCACCCUGCauucgcuaaucagUGGUGGUg -3' miRNA: 3'- -CGGCCa----CGcuAGUGGGACG-------------GCUACCA- -5' |
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2376 | 5' | -58.8 | NC_001416.1 | + | 7517 | 0.66 | 0.499699 |
Target: 5'- uGCCGGgaacgGCGuuUCGuguCUCUGCCGGUGuGg -3' miRNA: 3'- -CGGCCa----CGCu-AGU---GGGACGGCUAC-Ca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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