miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
2377 5' -57.1 NC_001416.1 + 7050 0.66 0.513904
Target:  5'- --gGGUGACCAccggcGAUCCGgCGCgUGAGu -3'
miRNA:   3'- aaaCUACUGGU-----CUGGGCgGCGgACUU- -5'
2377 5' -57.1 NC_001416.1 + 10066 0.67 0.441389
Target:  5'- --aGAggacGCCGGGCCCGCagaGCCUGu- -3'
miRNA:   3'- aaaCUac--UGGUCUGGGCGg--CGGACuu -5'
2377 5' -57.1 NC_001416.1 + 11367 1.04 0.000973
Target:  5'- gUUUGAUGACCAGACCCGCCGCCUGAAa -3'
miRNA:   3'- -AAACUACUGGUCUGGGCGGCGGACUU- -5'
2377 5' -57.1 NC_001416.1 + 15987 0.67 0.471794
Target:  5'- -gUGGUGAUgGGuaACCUGCCGCCg--- -3'
miRNA:   3'- aaACUACUGgUC--UGGGCGGCGGacuu -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.