Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2378 | 3' | -49.2 | NC_001416.1 | + | 14670 | 1.14 | 0.001671 |
Target: 5'- cCGAAUCACGCCGCAAUUUACUGCGGCg -3' miRNA: 3'- -GCUUAGUGCGGCGUUAAAUGACGCCG- -5' |
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2378 | 3' | -49.2 | NC_001416.1 | + | 14748 | 0.73 | 0.652009 |
Target: 5'- ----cCGuCGCCGCAGUaaAUUGCGGCg -3' miRNA: 3'- gcuuaGU-GCGGCGUUAaaUGACGCCG- -5' |
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2378 | 3' | -49.2 | NC_001416.1 | + | 5035 | 0.72 | 0.675059 |
Target: 5'- -aAAUCACGCUG--AUUUACaGCGGCa -3' miRNA: 3'- gcUUAGUGCGGCguUAAAUGaCGCCG- -5' |
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2378 | 3' | -49.2 | NC_001416.1 | + | 24144 | 0.71 | 0.751466 |
Target: 5'- ----aCACGUCGUAugcaggaacgUGCUGCGGCu -3' miRNA: 3'- gcuuaGUGCGGCGUuaa-------AUGACGCCG- -5' |
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2378 | 3' | -49.2 | NC_001416.1 | + | 366 | 0.69 | 0.86194 |
Target: 5'- aCGAAaagCuGCGCCGgGAgguugaagaACUGCGGCa -3' miRNA: 3'- -GCUUa--G-UGCGGCgUUaaa------UGACGCCG- -5' |
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2378 | 3' | -49.2 | NC_001416.1 | + | 9870 | 0.69 | 0.844226 |
Target: 5'- gCGuuUCugGUgGCGAUguccCUGUGGCa -3' miRNA: 3'- -GCuuAGugCGgCGUUAaau-GACGCCG- -5' |
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2378 | 3' | -49.2 | NC_001416.1 | + | 14144 | 0.69 | 0.834059 |
Target: 5'- uGGcgCACGCCcgGCGAUgUGCgccagcggagucgUGCGGCu -3' miRNA: 3'- gCUuaGUGCGG--CGUUAaAUG-------------ACGCCG- -5' |
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2378 | 3' | -49.2 | NC_001416.1 | + | 20275 | 0.68 | 0.884162 |
Target: 5'- uGGuggC-CGCCGCGuUUUuugaggacucugcgGCUGCGGCa -3' miRNA: 3'- gCUua-GuGCGGCGUuAAA--------------UGACGCCG- -5' |
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2378 | 3' | -49.2 | NC_001416.1 | + | 31987 | 0.68 | 0.867893 |
Target: 5'- cCGGuGUCAUGCUGCcaccuucugcuCUGCGGCu -3' miRNA: 3'- -GCU-UAGUGCGGCGuuaaau-----GACGCCG- -5' |
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2378 | 3' | -49.2 | NC_001416.1 | + | 3778 | 0.68 | 0.900016 |
Target: 5'- gCGuAUUACGCCGCAGcgccgguccgGCUGggaGGCg -3' miRNA: 3'- -GCuUAGUGCGGCGUUaaa-------UGACg--CCG- -5' |
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2378 | 3' | -49.2 | NC_001416.1 | + | 5674 | 0.68 | 0.901458 |
Target: 5'- ----gCGCGCCuguGCAcucuGUggUGCUGCGGCc -3' miRNA: 3'- gcuuaGUGCGG---CGU----UAa-AUGACGCCG- -5' |
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2378 | 3' | -49.2 | NC_001416.1 | + | 21040 | 0.68 | 0.901458 |
Target: 5'- uGGAUCAUuCC-CGAgc-GCUGCGGCc -3' miRNA: 3'- gCUUAGUGcGGcGUUaaaUGACGCCG- -5' |
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2378 | 3' | -49.2 | NC_001416.1 | + | 5843 | 0.68 | 0.894128 |
Target: 5'- cCGggUCACuGuuGCc-----CUGCGGCu -3' miRNA: 3'- -GCuuAGUG-CggCGuuaaauGACGCCG- -5' |
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2378 | 3' | -49.2 | NC_001416.1 | + | 5531 | 0.68 | 0.870404 |
Target: 5'- ---uUC-UGCCGCAAcc-GCUGCGGUg -3' miRNA: 3'- gcuuAGuGCGGCGUUaaaUGACGCCG- -5' |
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2378 | 3' | -49.2 | NC_001416.1 | + | 7884 | 0.68 | 0.878595 |
Target: 5'- ----cCACGCCGCA----GCUGCcGCa -3' miRNA: 3'- gcuuaGUGCGGCGUuaaaUGACGcCG- -5' |
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2378 | 3' | -49.2 | NC_001416.1 | + | 28470 | 0.67 | 0.93359 |
Target: 5'- uGAG-CAuUGCCGCAAUUUcuuUUGUGGUg -3' miRNA: 3'- gCUUaGU-GCGGCGUUAAAu--GACGCCG- -5' |
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2378 | 3' | -49.2 | NC_001416.1 | + | 33419 | 0.67 | 0.915222 |
Target: 5'- aCGAAUCAC-CCGUAAaUUACgUGaCGGa -3' miRNA: 3'- -GCUUAGUGcGGCGUUaAAUG-AC-GCCg -5' |
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2378 | 3' | -49.2 | NC_001416.1 | + | 16808 | 0.66 | 0.944311 |
Target: 5'- cCGGGggcaggCACaCCGCGccggGCUGUGGCu -3' miRNA: 3'- -GCUUa-----GUGcGGCGUuaaaUGACGCCG- -5' |
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2378 | 3' | -49.2 | NC_001416.1 | + | 39184 | 0.66 | 0.944311 |
Target: 5'- -----gAUGCUGCAAUUcAgaGCGGCa -3' miRNA: 3'- gcuuagUGCGGCGUUAAaUgaCGCCG- -5' |
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2378 | 3' | -49.2 | NC_001416.1 | + | 40057 | 0.66 | 0.953833 |
Target: 5'- gGAAUUACGCCGUAAg--GCcGCa-- -3' miRNA: 3'- gCUUAGUGCGGCGUUaaaUGaCGccg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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