Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23792 | 3' | -51.9 | NC_005261.1 | + | 84105 | 0.66 | 0.986882 |
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Target: 5'- aCGggggcGGGAugGGGAGGGGGGcagcgggcggggUCGCu -3' miRNA: 3'- gGCaga--CCCUugCUCUUCCUCU------------AGUG- -5' |
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| 23792 | 3' | -51.9 | NC_005261.1 | + | 33004 | 0.66 | 0.986882 |
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Target: 5'- gCCGaCcGGGGAaggGGGGAGGGGggCGCc -3' miRNA: 3'- -GGCaGaCCCUUg--CUCUUCCUCuaGUG- -5' |
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| 23792 | 3' | -51.9 | NC_005261.1 | + | 78410 | 0.66 | 0.987979 |
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Target: 5'- uCCGUgCUcugugggcgaugggGGGGGCGAuGGGGAGAagGCg -3' miRNA: 3'- -GGCA-GA--------------CCCUUGCUcUUCCUCUagUG- -5' |
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| 23792 | 3' | -51.9 | NC_005261.1 | + | 502 | 0.66 | 0.988427 |
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Target: 5'- -aGg--GGGAGgGAGAGGGGGGgagggCGCg -3' miRNA: 3'- ggCagaCCCUUgCUCUUCCUCUa----GUG- -5' |
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| 23792 | 3' | -51.9 | NC_005261.1 | + | 26828 | 0.66 | 0.988427 |
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Target: 5'- cCUGcUCUGGGAGgGGGGcgguGGGcGGUCAa -3' miRNA: 3'- -GGC-AGACCCUUgCUCU----UCCuCUAGUg -5' |
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| 23792 | 3' | -51.9 | NC_005261.1 | + | 138173 | 0.66 | 0.988427 |
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Target: 5'- -aGg--GGGAGgGAGAGGGGGGgagggCGCg -3' miRNA: 3'- ggCagaCCCUUgCUCUUCCUCUa----GUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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