Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23792 | 3' | -51.9 | NC_005261.1 | + | 134380 | 0.69 | 0.935847 |
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Target: 5'- gCCG-CUGGcGAcgGCGAGAGcGGGAUCAg -3' miRNA: 3'- -GGCaGACC-CU--UGCUCUUcCUCUAGUg -5' |
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| 23792 | 3' | -51.9 | NC_005261.1 | + | 32935 | 0.69 | 0.935847 |
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Target: 5'- gCGggggGGGGGCGGGGAGGGGAg--- -3' miRNA: 3'- gGCaga-CCCUUGCUCUUCCUCUagug -5' |
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| 23792 | 3' | -51.9 | NC_005261.1 | + | 13707 | 0.69 | 0.91965 |
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Target: 5'- gCGggCUGGGGGCGGGGGuGGGGGUgGg -3' miRNA: 3'- gGCa-GACCCUUGCUCUU-CCUCUAgUg -5' |
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| 23792 | 3' | -51.9 | NC_005261.1 | + | 57188 | 0.73 | 0.769368 |
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Target: 5'- -gGUCggcGGGAAgGGGggGGGGggCGCg -3' miRNA: 3'- ggCAGa--CCCUUgCUCuuCCUCuaGUG- -5' |
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| 23792 | 3' | -51.9 | NC_005261.1 | + | 110523 | 0.74 | 0.739901 |
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Target: 5'- gUCGUCUGcGGGGCGcAGGAGGGcGcgCGCa -3' miRNA: 3'- -GGCAGAC-CCUUGC-UCUUCCU-CuaGUG- -5' |
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| 23792 | 3' | -51.9 | NC_005261.1 | + | 22769 | 0.84 | 0.244798 |
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Target: 5'- gCGUCUGGGGgcgggccccGCGAGAAGGGGGuugUCGCg -3' miRNA: 3'- gGCAGACCCU---------UGCUCUUCCUCU---AGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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