Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23792 | 3' | -51.9 | NC_005261.1 | + | 15036 | 0.68 | 0.95788 |
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Target: 5'- gCCGg--GGGcguAGCGGGggGGgucgGGGUCGCg -3' miRNA: 3'- -GGCagaCCC---UUGCUCuuCC----UCUAGUG- -5' |
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| 23792 | 3' | -51.9 | NC_005261.1 | + | 13707 | 0.69 | 0.91965 |
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Target: 5'- gCGggCUGGGGGCGGGGGuGGGGGUgGg -3' miRNA: 3'- gGCa-GACCCUUGCUCUU-CCUCUAgUg -5' |
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| 23792 | 3' | -51.9 | NC_005261.1 | + | 11331 | 0.67 | 0.971269 |
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Target: 5'- -gGUg-GGGGugGGGAGGGAGggCAg -3' miRNA: 3'- ggCAgaCCCUugCUCUUCCUCuaGUg -5' |
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| 23792 | 3' | -51.9 | NC_005261.1 | + | 11080 | 0.66 | 0.98332 |
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Target: 5'- gCGUCUaGGGACGGGAcgGGGcGGGaCGCg -3' miRNA: 3'- gGCAGAcCCUUGCUCU--UCC-UCUaGUG- -5' |
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| 23792 | 3' | -51.9 | NC_005261.1 | + | 502 | 0.66 | 0.988427 |
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Target: 5'- -aGg--GGGAGgGAGAGGGGGGgagggCGCg -3' miRNA: 3'- ggCagaCCCUUgCUCUUCCUCUa----GUG- -5' |
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| 23792 | 3' | -51.9 | NC_005261.1 | + | 470 | 0.67 | 0.974072 |
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Target: 5'- gCCGgc-GGGAuggcGCGGGGAGGAGAg--- -3' miRNA: 3'- -GGCagaCCCU----UGCUCUUCCUCUagug -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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