Results 81 - 90 of 90 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23793 | 3' | -56.9 | NC_005261.1 | + | 59019 | 0.7 | 0.640917 |
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Target: 5'- ---cCAGCGGCgccGCCAGGAA-CGGGUg -3' miRNA: 3'- gcuaGUCGCCG---UGGUCCUUgGUCCAg -5' |
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| 23793 | 3' | -56.9 | NC_005261.1 | + | 93675 | 0.7 | 0.640917 |
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Target: 5'- gCGggCGGCGGCGCCGGcGGggcGCCggcGGGUg -3' miRNA: 3'- -GCuaGUCGCCGUGGUC-CU---UGG---UCCAg -5' |
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| 23793 | 3' | -56.9 | NC_005261.1 | + | 107566 | 0.7 | 0.650155 |
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Target: 5'- aCGG-CAGCGGCGaagUAGGGcgcgaagGCCGGGUCc -3' miRNA: 3'- -GCUaGUCGCCGUg--GUCCU-------UGGUCCAG- -5' |
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| 23793 | 3' | -56.9 | NC_005261.1 | + | 69764 | 0.7 | 0.651181 |
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Target: 5'- gCGGggGGCGGCGCCGGGGACUg---- -3' miRNA: 3'- -GCUagUCGCCGUGGUCCUUGGuccag -5' |
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| 23793 | 3' | -56.9 | NC_005261.1 | + | 13145 | 0.69 | 0.658358 |
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Target: 5'- uCGGUgaCGGCGcGCACCAGGcgguccgaggugacGACCAGGc- -3' miRNA: 3'- -GCUA--GUCGC-CGUGGUCC--------------UUGGUCCag -5' |
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| 23793 | 3' | -56.9 | NC_005261.1 | + | 103858 | 0.69 | 0.660406 |
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Target: 5'- gCGAgccgCGGCGGCGgCGGGGcggcgccGCCGGG-Cg -3' miRNA: 3'- -GCUa---GUCGCCGUgGUCCU-------UGGUCCaG- -5' |
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| 23793 | 3' | -56.9 | NC_005261.1 | + | 65670 | 0.69 | 0.66143 |
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Target: 5'- gCGcgCAGCGcCGCCAGc-ACCGGGUCc -3' miRNA: 3'- -GCuaGUCGCcGUGGUCcuUGGUCCAG- -5' |
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| 23793 | 3' | -56.9 | NC_005261.1 | + | 72839 | 0.69 | 0.66143 |
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Target: 5'- gCGGUCGGCcagGGCGCCAGcgcCCAGG-Cg -3' miRNA: 3'- -GCUAGUCG---CCGUGGUCcuuGGUCCaG- -5' |
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| 23793 | 3' | -56.9 | NC_005261.1 | + | 92633 | 0.69 | 0.671655 |
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Target: 5'- gCGAg-AGCGGgACCGGGA-CCGGGa- -3' miRNA: 3'- -GCUagUCGCCgUGGUCCUuGGUCCag -5' |
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| 23793 | 3' | -56.9 | NC_005261.1 | + | 92473 | 0.68 | 0.722116 |
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Target: 5'- gGAUgAGCGGCACCGcgagcucgcGGAcggcgGCCAGcucGUCa -3' miRNA: 3'- gCUAgUCGCCGUGGU---------CCU-----UGGUC---CAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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