Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 23795 | 3' | -53.9 | NC_005261.1 | + | 48901 | 0.66 | 0.939798 |
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Target: 5'- cGCAguUGGcCUCUAGCGCgagCCcgGGCGc -3' miRNA: 3'- -CGU--AUCuGAGGUCGCGga-GGuaCUGC- -5' |
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| 23795 | 3' | -53.9 | NC_005261.1 | + | 41114 | 0.66 | 0.939798 |
|
Target: 5'- cGCGgcGGGC-CCgAGCGCUUCCugcGUGGCGc -3' miRNA: 3'- -CGUa-UCUGaGG-UCGCGGAGG---UACUGC- -5' |
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| 23795 | 3' | -53.9 | NC_005261.1 | + | 31862 | 0.66 | 0.939798 |
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Target: 5'- cGCGUcuucGGGC-CCGGCGCCUUCGcGcGCGc -3' miRNA: 3'- -CGUA----UCUGaGGUCGCGGAGGUaC-UGC- -5' |
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| 23795 | 3' | -53.9 | NC_005261.1 | + | 57565 | 0.66 | 0.93487 |
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Target: 5'- cGCGcccGGCUCCGGCGCCgCguUGAgCGc -3' miRNA: 3'- -CGUau-CUGAGGUCGCGGaGguACU-GC- -5' |
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| 23795 | 3' | -53.9 | NC_005261.1 | + | 13435 | 0.66 | 0.93487 |
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Target: 5'- cGCGUcgaccgggugcAGGCaggCCGGCGCCUCgGgGugGa -3' miRNA: 3'- -CGUA-----------UCUGa--GGUCGCGGAGgUaCugC- -5' |
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| 23795 | 3' | -53.9 | NC_005261.1 | + | 76054 | 0.66 | 0.957069 |
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Target: 5'- aGCGUGGcCggCgGGCGCCaCCAgGGCGg -3' miRNA: 3'- -CGUAUCuGa-GgUCGCGGaGGUaCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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