Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23795 | 3' | -53.9 | NC_005261.1 | + | 62314 | 0.66 | 0.94448 |
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Target: 5'- cGCGgcGGCcccgcggucccCCAGCGCCUCCAccGCGc -3' miRNA: 3'- -CGUauCUGa----------GGUCGCGGAGGUacUGC- -5' |
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| 23795 | 3' | -53.9 | NC_005261.1 | + | 34933 | 0.66 | 0.94448 |
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Target: 5'- cGCGggGGGCggCCGGCGCgaCCcgGGCc -3' miRNA: 3'- -CGUa-UCUGa-GGUCGCGgaGGuaCUGc -5' |
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| 23795 | 3' | -53.9 | NC_005261.1 | + | 123344 | 0.66 | 0.948485 |
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Target: 5'- aGCGgcucgcGGCcgaCCAGCGCCUCUAUGcuguccaGCGa -3' miRNA: 3'- -CGUau----CUGa--GGUCGCGGAGGUAC-------UGC- -5' |
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| 23795 | 3' | -53.9 | NC_005261.1 | + | 53276 | 0.66 | 0.953113 |
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Target: 5'- aGCAUgucgauggAGGCgCCaAGCGCCcCCGUGcGCGg -3' miRNA: 3'- -CGUA--------UCUGaGG-UCGCGGaGGUAC-UGC- -5' |
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| 23795 | 3' | -53.9 | NC_005261.1 | + | 126264 | 0.66 | 0.956684 |
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Target: 5'- cGCGUccaAGAagccgucCUCCAGCGCCacgacgCCGUGGu- -3' miRNA: 3'- -CGUA---UCU-------GAGGUCGCGGa-----GGUACUgc -5' |
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| 23795 | 3' | -53.9 | NC_005261.1 | + | 76054 | 0.66 | 0.957069 |
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Target: 5'- aGCGUGGcCggCgGGCGCCaCCAgGGCGg -3' miRNA: 3'- -CGUAUCuGa-GgUCGCGGaGGUaCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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