Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23795 | 3' | -53.9 | NC_005261.1 | + | 31862 | 0.66 | 0.939798 |
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Target: 5'- cGCGUcuucGGGC-CCGGCGCCUUCGcGcGCGc -3' miRNA: 3'- -CGUA----UCUGaGGUCGCGGAGGUaC-UGC- -5' |
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| 23795 | 3' | -53.9 | NC_005261.1 | + | 14719 | 0.7 | 0.805647 |
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Target: 5'- gGCcgAGAUUCCAGCGUCacgaCgGUGGCa -3' miRNA: 3'- -CGuaUCUGAGGUCGCGGa---GgUACUGc -5' |
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| 23795 | 3' | -53.9 | NC_005261.1 | + | 13435 | 0.66 | 0.93487 |
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Target: 5'- cGCGUcgaccgggugcAGGCaggCCGGCGCCUCgGgGugGa -3' miRNA: 3'- -CGUA-----------UCUGa--GGUCGCGGAGgUaCugC- -5' |
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| 23795 | 3' | -53.9 | NC_005261.1 | + | 11062 | 0.7 | 0.796524 |
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Target: 5'- cGCccAGGCgccgCCAGCGCgUCUAggGACGg -3' miRNA: 3'- -CGuaUCUGa---GGUCGCGgAGGUa-CUGC- -5' |
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| 23795 | 3' | -53.9 | NC_005261.1 | + | 2279 | 0.71 | 0.768262 |
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Target: 5'- cGCcgGGcgGCgccgCCAGCGCCUCCccgGGCa -3' miRNA: 3'- -CGuaUC--UGa---GGUCGCGGAGGua-CUGc -5' |
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| 23795 | 3' | -53.9 | NC_005261.1 | + | 639 | 0.68 | 0.872034 |
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Target: 5'- gGCGgcGGCcgCCAGCGCCgcgUCCccGGCGc -3' miRNA: 3'- -CGUauCUGa-GGUCGCGG---AGGuaCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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