Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23795 | 3' | -53.9 | NC_005261.1 | + | 80579 | 0.7 | 0.796524 |
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Target: 5'- ---cAGGCUCCuugaGGCGCCggcCCGUGGCc -3' miRNA: 3'- cguaUCUGAGG----UCGCGGa--GGUACUGc -5' |
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| 23795 | 3' | -53.9 | NC_005261.1 | + | 11062 | 0.7 | 0.796524 |
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Target: 5'- cGCccAGGCgccgCCAGCGCgUCUAggGACGg -3' miRNA: 3'- -CGuaUCUGa---GGUCGCGgAGGUa-CUGC- -5' |
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| 23795 | 3' | -53.9 | NC_005261.1 | + | 74562 | 0.7 | 0.793756 |
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Target: 5'- gGCGUGGACaUCCAcgcggucgcggaccGCGUCUUCGUGcCGg -3' miRNA: 3'- -CGUAUCUG-AGGU--------------CGCGGAGGUACuGC- -5' |
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| 23795 | 3' | -53.9 | NC_005261.1 | + | 40569 | 0.7 | 0.777822 |
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Target: 5'- -------gUCCAGCGCCUCCAcgaacucgcUGACGu -3' miRNA: 3'- cguaucugAGGUCGCGGAGGU---------ACUGC- -5' |
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| 23795 | 3' | -53.9 | NC_005261.1 | + | 121977 | 0.7 | 0.777822 |
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Target: 5'- -----cGCUCCAGCGCCgcCCAgcUGGCGg -3' miRNA: 3'- cguaucUGAGGUCGCGGa-GGU--ACUGC- -5' |
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| 23795 | 3' | -53.9 | NC_005261.1 | + | 48853 | 0.79 | 0.319398 |
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Target: 5'- cGCAgcAGGC-CCAGCGCCcgggCCAUGGCGg -3' miRNA: 3'- -CGUa-UCUGaGGUCGCGGa---GGUACUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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