Results 1 - 20 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23807 | 3' | -56.3 | NC_005261.1 | + | 97359 | 0.69 | 0.723906 |
Target: 5'- cCGUGGGGucuacGGCCCgg-GCGUGucgccGCGCg -3' miRNA: 3'- uGCACCUU-----CCGGGacaUGCACu----CGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 17082 | 0.66 | 0.894468 |
Target: 5'- gACG-GGAuGGGCCCgGUG-GUGugccAGCGCu -3' miRNA: 3'- -UGCaCCU-UCCGGGaCAUgCAC----UCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 40226 | 0.68 | 0.79989 |
Target: 5'- aGCGUcGucgucucuGGCCCgggGgcCGUGAGCGCc -3' miRNA: 3'- -UGCAcCuu------CCGGGa--CauGCACUCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 68281 | 0.68 | 0.790832 |
Target: 5'- cGCGcGGcGGGGCCCcGgcCGcGGGCGCg -3' miRNA: 3'- -UGCaCC-UUCCGGGaCauGCaCUCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 43786 | 0.68 | 0.790832 |
Target: 5'- cGCGcGGAGGGCgCCgcggGUGaggGGGCGCc -3' miRNA: 3'- -UGCaCCUUCCG-GGa---CAUgcaCUCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 61576 | 0.68 | 0.78163 |
Target: 5'- gGCGcUGGugauGGCCgUGUuCGUGGGCaGCc -3' miRNA: 3'- -UGC-ACCuu--CCGGgACAuGCACUCG-CG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 49361 | 0.68 | 0.772293 |
Target: 5'- cGCGUGGAggauguugAGGCUgucgcucgcgCUGgccaGCGUGcAGCGCu -3' miRNA: 3'- -UGCACCU--------UCCGG----------GACa---UGCAC-UCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 17178 | 0.68 | 0.772293 |
Target: 5'- gGCGUGGGcaGCCCUGccgcgGCGgcgaGcAGCGCg -3' miRNA: 3'- -UGCACCUucCGGGACa----UGCa---C-UCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 43609 | 0.68 | 0.76283 |
Target: 5'- gGCGaGGAGGGCgagugCUUcGUGCuGUGGGCGCc -3' miRNA: 3'- -UGCaCCUUCCG-----GGA-CAUG-CACUCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 58680 | 0.69 | 0.743564 |
Target: 5'- uACGgcaGGAGGCCCgGgcCGUcaGGGCGCg -3' miRNA: 3'- -UGCac-CUUCCGGGaCauGCA--CUCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 89771 | 0.69 | 0.723906 |
Target: 5'- cCGUGGccgagGAGGCCCU--GCGgGAGCGg -3' miRNA: 3'- uGCACC-----UUCCGGGAcaUGCaCUCGCg -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 31556 | 0.76 | 0.362861 |
Target: 5'- cACGccUGGGccGGGUCCUGgacgggcgcgccggACGUGAGCGCg -3' miRNA: 3'- -UGC--ACCU--UCCGGGACa-------------UGCACUCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 118961 | 0.69 | 0.713953 |
Target: 5'- cACGUGGccAAGGUCCUGcACGcc-GCGCu -3' miRNA: 3'- -UGCACC--UUCCGGGACaUGCacuCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 98364 | 0.7 | 0.674551 |
Target: 5'- -gGUGGGcgcGGGCUCUGgcgggcgcaauaccgGCGUGGGgGCg -3' miRNA: 3'- ugCACCU---UCCGGGACa--------------UGCACUCgCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 58014 | 0.7 | 0.673532 |
Target: 5'- gACGUGGAAGuGCCgCg--GCGgcagcuccaggGAGCGCg -3' miRNA: 3'- -UGCACCUUC-CGG-GacaUGCa----------CUCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 88469 | 0.7 | 0.663319 |
Target: 5'- gUGUGGGcGGGCCgUGccGCGccgGAGCGCg -3' miRNA: 3'- uGCACCU-UCCGGgACa-UGCa--CUCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 105578 | 0.7 | 0.653081 |
Target: 5'- cGCGUaGAGGGCCUcgGggcGCGUGcGCGCg -3' miRNA: 3'- -UGCAcCUUCCGGGa-Ca--UGCACuCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 130482 | 0.71 | 0.6223 |
Target: 5'- gGCGUGccucggcggcGAGGGCCCcGacgACGUG-GCGCa -3' miRNA: 3'- -UGCAC----------CUUCCGGGaCa--UGCACuCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 22102 | 0.72 | 0.551077 |
Target: 5'- gGCG-GGAGGGCggUGUGCGgaGAGUGCg -3' miRNA: 3'- -UGCaCCUUCCGggACAUGCa-CUCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 120619 | 0.75 | 0.39844 |
Target: 5'- cCGUGGGGcuccugcugguggcGGCCCUGUGCcUGcGGCGCa -3' miRNA: 3'- uGCACCUU--------------CCGGGACAUGcAC-UCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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