Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23809 | 3' | -53.2 | NC_005261.1 | + | 16385 | 0.66 | 0.969683 |
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Target: 5'- -cGUGGgcc-UCGCCGUcgucGUCGCCGGg -3' miRNA: 3'- caCACCaucuAGCGGCAc---UAGUGGCU- -5' |
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| 23809 | 3' | -53.2 | NC_005261.1 | + | 74816 | 0.66 | 0.96656 |
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Target: 5'- -cGUGGcGGAgguggcCGCCGUGcUCGCgGAc -3' miRNA: 3'- caCACCaUCUa-----GCGGCACuAGUGgCU- -5' |
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| 23809 | 3' | -53.2 | NC_005261.1 | + | 62762 | 0.67 | 0.94753 |
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Target: 5'- cUGUGGgagAGGUCGCCGgggagcacGAUCuCCa- -3' miRNA: 3'- cACACCa--UCUAGCGGCa-------CUAGuGGcu -5' |
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| 23809 | 3' | -53.2 | NC_005261.1 | + | 75512 | 0.67 | 0.93824 |
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Target: 5'- --cUGG-AGGcgcaCGCCGUGGUCGCCGc -3' miRNA: 3'- cacACCaUCUa---GCGGCACUAGUGGCu -5' |
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| 23809 | 3' | -53.2 | NC_005261.1 | + | 46116 | 0.68 | 0.897897 |
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Target: 5'- -cGUGGUccgcguccagcAGGUCGCCGcccGAgaaCGCCGAg -3' miRNA: 3'- caCACCA-----------UCUAGCGGCa--CUa--GUGGCU- -5' |
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| 23809 | 3' | -53.2 | NC_005261.1 | + | 88468 | 0.69 | 0.89115 |
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Target: 5'- cGUGUGGgcGG--GCCGUGccgCGCCGGa -3' miRNA: 3'- -CACACCauCUagCGGCACua-GUGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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