miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
2381 3' -53.8 NC_001416.1 + 16004 1.08 0.001432
Target:  5'- gCCGCCGCGCCCGUUUAAUAUCCGGAUg -3'
miRNA:   3'- -GGCGGCGCGGGCAAAUUAUAGGCCUA- -5'
2381 3' -53.8 NC_001416.1 + 4349 0.66 0.759155
Target:  5'- aCCGCC-CGCCUGggcggcugcagcaUUUGA-AUCCGGGc -3'
miRNA:   3'- -GGCGGcGCGGGC-------------AAAUUaUAGGCCUa -5'
2381 3' -53.8 NC_001416.1 + 4657 0.66 0.78074
Target:  5'- aUCGCCGUGCugCCG----GUGUCCGGc- -3'
miRNA:   3'- -GGCGGCGCG--GGCaaauUAUAGGCCua -5'
2381 3' -53.8 NC_001416.1 + 2434 0.66 0.74549
Target:  5'- gCUGCUGCGCuUCGgucagaucaaaAAUAUCCGGGUu -3'
miRNA:   3'- -GGCGGCGCG-GGCaaa--------UUAUAGGCCUA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.